摘要
目的:探索未获培养微生物与牙周可疑致病菌的共生关系。方法:使用基于16S rRNA基因检测的PCR法,对慢性牙周炎患者龈下菌斑中牙周可疑致病微生物和代表性未获培养微生物进行检测,通过KAPPA检验分析任意两种细菌对间的关系。结果:在56个细菌对中发现6对细菌的KAPPA值具有显著性。结论:P.g/T.d、B.f/T.d、B.f/F.n细菌组合的共生关系与文献报道一致,AU126/P.n、AU126/F.n、AU126/X112细菌对间的共生关系显示了未获培养微生物在龈下菌群中扮演的角色。
AIM：To find the symbiotic relationship existed between unculturable phylotypes ＆ putative periodontal pathogens. METHODS ： The suspected pathogens and unculturable phylotypes in subgingival oral flora from chronic periodontitis sufferer were detected by PCR based on species - specific ribosomal 16S rRNA gene. A KAPPA calculation was used to analyze the relationship between any two bacterial species. RESULTS：Computation of KAPPA indicated a significant positive association for 6 of the 56 bacterial pairs. CONCLUSION：Symbiotic relationship of P. g/T. d,B.f/T, d,B,f/F, n combinations accorded with other＇s conclusions, Symbiotic relationship of AU126/P. n, AU126/F. n,AU126/X112 showed the unculturable phylotypes＇s roles in the subgingival oral flora.
出处
《牙体牙髓牙周病学杂志》
CAS
2006年第1期24-27,共4页
Chinese Journal of Conservative Dentistry
基金
上海市教育发展基金资助(05048)
关键词
未获培养
牙周可疑致病菌
共生关系
牙周
uncultivated
putative periodontal pathogen
Symbiotic relationship
