摘要
应用3′RACE法降落式扩增并克隆了猪水泡病病毒(SVDV)HK/70株基因组3′端的真实序列。测序结果表明,所扩增的目的基因片段长4 200 nt,包括非结构蛋白编码区(P2和P3)、3′端非编码区和poly(A)尾,其中3′NTR位于终止密码子TAA之后,长99 nt,poly(A)至少含有74个腺嘌呤碱基。经与参考毒株进行序列比较,结果显示,HK/70株与J1′73、H/3′76和AUG/22/90株的核苷酸同源性较高,为99.0%(仅第65位碱基不同),而与NET/1/92参考毒株的核苷酸同源性较低,为83.3%,与人柯萨奇病毒B5 UK/54株(UK/54-CB5)的同源性为75.7%。同时对3′NTR的二级结构进行分析,并与参考毒株和CB5毒株的二级结构进行了比较。结果表明,它们具有协同变异性,有共同的祖先,显示出3′NTR存在支持其功能所必需的一些结构域。
The authentic 3＇end sequence, including P1, P2, 3＇NTR, and poly （A） tail, was amplified by 3＇RACE, and the results showed that 3＇NTR was 99 nt in length, and the poly（A） tail had at least had 74 adenines. Compared with other reference strains, HK/70 strain shared higher nucleotide acid identity with J1＇73, H/3＇76 and AUG/22/90 strains（99.0%） than with NET/2/92 and UK/54-CBS（83.3% and 75.7%）, The secondary structure of HK/70 strain was predicted and analyzed by RNAdraw software, and results showed that there were some special domains in the 3＇NTR, which was necessary for its biological function, and there are also some covariable positions supporting the hypothesis which SVDV most likely arose from a single common ancestor. SVDV may be a recently evolved genetic subspecies of CB5.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第1期7-12,共6页
Chinese Veterinary Science
基金
国家高技术研究发展计划(863)项目(2003AA241110)
