摘要
目的 目的是利用阿仑膦酸钠来研究其对人工关节假体周围骨溶解的影响及其作用机制。方法 体重300～350g的雄性SD大鼠24只，经膝关节将特制的钛合金假体及混合磨损颗粒植入胫骨近端（双侧），随机分为实验组和对照组，每组12只，术后分别每日空腹阿仑膦酸钠（0.1mg／kg体重）灌胃和生理盐水2ml灌胃，共6周。术后12周处死取材，进行组织学观察及组织形态计量学测定，并采用ELISA法及半定量RT—PCR检测假体周围组织中TNF-α的的含量及OPGL／OPG含量的比率。结果 组织学观察发现，对照组假体柄周围纤维界膜厚、细胞成分多。可分3层：紧贴假体处为疏松结缔组织，稍外为致密纤维结缔组织。最外层含有单核细胞、巨噬细胞及异物巨细胞。与纤维界膜连接处新生骨边缘呈虫蚀状，新生骨与假体接触少。对假体的支撑作用差。实验组假体周围纤维界膜较薄、细胞成分少，多为成纤维细胞。新生骨与假体间呈点状接触。对假体有明显的支撑作用。形态计量学检测发现，两组间假体周围界膜厚度及面积差异有统计学意义；ELISA和半定量RT—PCR检测假体周围组织中TNF-α及OPGL／OPG发现。两组间差异有统计学意义。结论 阿仑膦酸钠不仅可直接作用于假体周围组织的中破骨细胞。同时可通过调节假体周围组织分泌TNF-α、OPGL、OPG等的含量来调节破骨细胞的分化、成熟及增殖。
Objective To observe the effects and to investigate the mechanism of alendronate on the periprosthetical osteolysis. Methods The specially designed implants made of Ti alloy and the mixed wear particles were implanted into the proximal part of bilateral tibias of 24 SD rots. Then the rats were randomized into the ：ontrol group（12 rats） and the experimental group（12 rats）. In the eontrol group, the saline was perfused into the empty stomach daily for 6 weeks;in the experimental group, alendronate, 0.1 mg/kg/day, was perfused into the empty stomach daily for 6 weeks.At 12 week postoperatively, the rats were sacrificed with the overdose anesthesia and all of the tibias were harvested. Then the histology and the histomorphometry were carried out. TNF-α and OPGL/OPG in the periprosthetical tissue were respectively tested by the ELISA and the semi-quantiffed RT-PCR. Results The histological analysis showed that there were fibrous membrane and the newly formed bone around the implant in the eontrol group. The fibrous membrane was thick with large numbers of cells. The fibrous membrane can be divided into three areas, as following：loose eonnective tissue dose to the prosthesis, fibrous eonnective tissue and outer area which contains the monocytes, macrophages and foreign body giant cells et al. The interface between the fibrous membrane and the newly formed bone is eclipsed. The newly formed bone cannot support the prosthesis. In the experimental group, the histological analysis showed that there were fibrous membrane and newly formed bone around the implant. The fibrous membrane was rare and thin with few cells. It could be seen that the newly formed bone eould support the prosthesis. Thickness and area of the fibrous membrane around the implant tested by the histomorphometry demonstrated that it was significant the difference between the control group and the experimental group in statistics. It showed that the difference was significant in statistics when TNF-α and OPGL/OPG in the periprosthetical tissue
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第2期154-156,共3页
Chinese Journal of Experimental Surgery
