摘要
目的观察还原型谷胱甘肽(glutath ione,GSH)对硫芥(su lfurmustard,SM)诱导大鼠脾淋巴细胞线粒体功能的保护作用。方法用密度梯度离心法分离大鼠脾淋巴细胞,与硫芥共同进行体外培养。用MTT法检测线粒体功能状态;用荧光分光法检测细胞内GSH含量和用琼脂糖DNA凝胶电泳观察DNA的损伤。结果硫芥作用的线粒体功能变化具有剂量和时间依赖性,它与细胞内GSH含量下降呈直线正相关(P<0.01)。使用GSH后,对不同剂量组的线粒体功能早期均有保护作用。保护效果最好时间的DNA断裂情况有所减轻,甚至可以逆转坏死向凋亡发展。结论GSH可部分保护线粒体功能。
Objective To investigate the protective effects of GSH on the mitochondria in rat lymphocyte in vitro. Methods Rat spleen lymphocytes were isolated by density gradient centrifugation and cultured with sulfur mustard of 100, 500, 1000 μmol/L in vitro. The mitochondria function was detected by MTF. The changes of GSH were detected by fluorescence spectrophotometry and DNA fragments were also detected. Results There was a positive line correlation between the changes of mitochondria function and the GSH content (P 〈 0. 01 ). GSH could protect the mitochondria function in the lymphocytes treated with sulfur mustard. GSH could alleviate the apoptosis, even change the necrosis to apoptosis sometimes. Conclusion GSH can maintain the mitochondria function and alleviate the apoptosis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第1期63-65,共3页
Journal of Third Military Medical University
基金
全军医学科学技术研究"十五"计划指令性课题(01L054)~~