摘要
目的探讨携带血管生成素基因(Ang1)的骨髓间质干细胞(MSCs)对股骨头缺血性坏死(ANFH)的修复作用。材料与方法将80只造模兔随机分为钻孔注入组、动脉灌注组、双介入组、对照组,实验组采用经皮穿刺股骨头钻孔,直接注入携带Ang1的MSCs,和/或经颈动脉穿刺,行超选择插管至双侧股骨头供血动脉,缓慢注入携带Ang1的MSCs,对照组不作处理,术后2、4、6、8周分别进行DR、CT、MRI、DSA检查,观察骨质及血管情况。结果(1)DR显示实验组股骨头密度增高,尤以双介入组明显,高于同期对照组。(2)CT、MRI灌注成像见实验组4周后血流量增多,尤以双介入组明显,多于同期对照组。(3)DSA可见实验组的新生血管及侧支循环数目增多,尤以双介入组明显,多于同期对照组。结论携带Ang1的MSCs经双介入治疗对ANFH有明显的修复作用。
Objective To study the possible therapeutic effect of mesenchymalstem cells with Angiopoietin gene expression in the treatment of corticosteroid induced avascular necrosis of the femoral head （ANFH）. Materials and Methods 80 models of rabbits were randomly assigned into drill group, artery perfusion group, double interventional group and controul group, Msenchymalstem cells with angiopoietin genewere were taken into by drill or superselective catheter to feeding arteries of femoral head in experimental group. 80 models of bilateral femoral head were examined by DR, CT, MRI and DSA at 2,4,6,8 weeks after the double （dual） interventional therapy. The effect of the treatment on the bones and the blood vessels were observed. Results （1） Analysis of DR value revealed that the bone density was significantly increased in the double （dual） interventional therapy group compared with that of the other groups. （2） CT and MR perfusion imaging demonstrated hemodynamic status of femoral head four weeks after the injection, blood flow to the femoral heads began increasing in the test group compared with that of the other groups. （3） DSA revealed an increase in the number of new blood vessels and collateral branches in the double （dual） interventional therapy group compared with that of the other groups. Conclusion Mesenchymal stem cells with angiopoietin gene expression has apparent promotional effect on the repairing process of ANFH after the double（dual） interventional therapy.
出处
《临床放射学杂志》
CSCD
北大核心
2006年第1期79-82,共4页
Journal of Clinical Radiology
基金
湖北省教育厅科学技术研究项目(N0.B200528002)
关键词
股骨头坏死
介入治疗
血管生成素
骨髓间质干细胞
修复
Femoral head necrosis Interventional therapy Angiopoietin-gene Mesenchymal stem cells Repairing
