摘要
对厚荚相思(Acaciacrassicarpa)根瘤菌HJ06菌株的16SrDNA全序列和nifA基因片段进行了测定。结果表明,HJ06菌株在以16SrDNA序列构建的系统发育树状图中位于根瘤菌属(Rhizobium)分支中,与根瘤菌属各个种的相似性达95%以上;从HJ06菌株克隆出的585bpnifA基因片段与Klebsiellapneumoniae的同源性达到99.3%,与Klebsiellaoxytoca的NifF,NifL,NifA,NifB蛋白基因的同源性为97.8%。
The determination of full-length 16S rDNA sequence and nifA gene segments of root nodule bacterium strain HJ06 isolated from Acacia crassicarpa showed that strain HJ06 had above 95% similarity with the genus Rhizobium. The nifA gene segments of HJ06 have 99.3% isogenesis with nifA genes of Klebsiella pneumoniae and 97.8% isogenesis with genes ofNifF, NifL, NifA and NifB proteins of K. oxytoca.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2006年第1期19-24,共6页
Journal of Tropical and Subtropical Botany
基金
广西科学基金项目(桂科基0448005)
广西"十五"林业科学研究项目(林科字2002第19号)资助
