摘要
通过FX-4000基底加载系统研究了在不同波形、频率、循环数及拉伸幅度下周期性拉伸应变对人肺腺癌 A549细胞株生长增殖的影响,应用Image-pro软件对A549细胞的增殖动力学变化进行了分析．实验表明: (1)在laminin基底膜上,应变0％-15％,加载时间2 h,方波拉伸组细胞增殖率(PR)明显降低,正弦波、心形波及三角波拉伸组细胞PR与对照组比较无明显差异;(2)方波作用下,0．5Hz及1．0Hz频率组细胞增殖明显受到抑制,0．3Hz及0．7Hz频率组细胞PR与对照组比较无明显差异; (3)方波作用下,幅度为15％, 20％及25％的拉伸组与对照组比较细胞PR均无明显差异．研究表明: A549细胞株对体外的生理应变作出响应时,方波作用下1．0 Hz频率组的抑制作用最明显,且加载时间越长抑制作用越强．
The effects of waveform, frequency, cycle number and elongation on proliferation of human lung adenocarcinoma cell line A549 were studied by FX-4000 strain unit. Image analysis revealed that cellular proliferation rate（PR） reduced significantly at squarewave group, The PR had no distinct difference at heartwave, trianglewave and sinewave group compared with control after A549 cells were subjected to 0%-15% elongation for 2 h. The PR reduced significantly at 0.5 Hz and 1.0 Hz group, The PR had no distinct difference at 0.3 Hz and 0.7 Hz group after A549 cells were subjected to squarewave for 2 h. The PR had no distinct difference at 15%, 20% and 25% elongation for squarewave group. It indicates that A549 cells do respond to physiological strain in vitro. The effects of squarewave and 1.0 Hz tension was distinct for A549 cells proliferation.
出处
《力学学报》
EI
CSCD
北大核心
2006年第1期130-133,共4页
Chinese Journal of Theoretical and Applied Mechanics
基金
国家自然科学基金重点项目(10332060)山西省青年科学基金资助项目(20041002).~~
关键词
周期性拉伸
波形
频率
循环数
拉伸幅度
A549细胞株
增殖
mechanical strain, waveform, frequency, cycle number, elongation, A549 cells, proliferation
