摘要
目的研究线粒体DNA(mtDNA)编码区单核苷酸多态性,建立mtDNA编码区多态性在法庭科学中应用的理论基础。方法针对mtDNA编码区nt8162-8483以及nt13070-13299两段序列设计引物,应用直接测序技术研究其多态性。结果两对引物扩增片段长分别为322bp和230bp,共检测到21种变异,24种单倍型,基因多样性为0.7511,两个无关个体的偶合概率为0.2564。结论线粒体DNA编码区多态性位点作为线粒体DNA控制区多态性位点的补充,联合应用可以提高线粒体DNA在法医学应用中的个体识别能力。
Objective To analyze mitochondrial DNA （mtDNA） polymorphisms in coding area and provide a theoretical basis for applying in forensic science. Methods The primersof 8162F/8483R and 13070F/13299R were designed according to the Anderson＇s sequence. Using PCR-sequencing method to detect polymorphisms of mtDNA nt8162-8483 and ntl3070-13299. Results The lengths of the amplicons were 322 bp and 230 bp respectively. There were 24 mitochondrial haplotypes defined by 21 variable positions in both regions. The gene diversity was estimated at 0.751 1, and the probability of two randomly selected individuals having identical mtDNA types was 0.256 4. Conclusion The polymorphic sites within mtDNA coding area can be useful in combination with mtDNA control region in order to increase the discrimination power.
出处
《法医学杂志》
CAS
CSCD
2006年第1期45-47,共3页
Journal of Forensic Medicine
关键词
线粒体DNA编码区
单核苷酸多态性
测序
mitochondrial DNA
coding area
single nucleotide polymorphisms
sequencing
