摘要
用RT-PCR扩增小鼠巨噬细胞源趋化因子(macrophage-derived chemokine,MDC)基因,与柯萨奇病毒B组3型(CVB3)VP1基因通过一个编码10个氨基酸残基的接头序列(Gly4Ser)2相连,形成融合基因MDC-VP1,构建真核表达质粒pcDNA3/MDC-VP1,作为疫苗免疫BALB/c小鼠.3次免疫后,pcDNA3/MDC-VP1组小鼠血清中和抗体滴度明显高于pcDNA3/VP1组;而病毒滴度低于pcDNA3/VP1组.用10 LD50CVB3攻击,pcDNA3/MDC-VP1组小鼠生存率为50%,用Kaplan-Meier法进行生存分析,生存率高于其他各组.
Macrophage-derived chemokine （MDC） gene fragment was amplified by RT-PCR, and linked to Coxsackievirus B3 （CVB3） VP1 gene to construct an eukaryotic expressing plasmid pcDNA3/MDC-VP1 by a DNA sequence encoding a flexible polypeptide （10 amino acids）. The plasmid was inoculated to mice as a vaccine. After the third inoculation, the mean neutralizing antibody titer in pcDNA3/MDC-VP1 group was higher than that of pcDNA3/VP1, and the virus titer of blood in pcDNA3/MDC-VP1 group was lower than that of pcDNA3/VP1. After 10 LD50 CVB3 challenge, the survival rate of pcDNA3/MDC-VP1 group was 50 %, Kaplan-Meier test was used to compare differences of survival curves, the results showed that the survive state of pcDNA3/MDC-VP1 group is better than that of other groups （P〈0.05）.
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2006年第1期16-20,共5页
Journal of Hebei University(Natural Science Edition)
基金
河北省自然科学基金资助项目(C2004000631)
