摘要
AIM： To investigate the effect of cyclosporine A （CsA）, FK-506, and mycophenolate mofetil （MMF） and 40-0-[2- hydroxyethyl]rapamycin （RAD） on proliferation of human intrahepatic biliary epithelial cells （BECs） in vitro.METHODS： BECs were isolated from six human liver tissuespecimens with the immunomagnetic separation method and treated with different concentrations of CsA, FK-S06, RAD, and MMF in vitro. Proliferation of the cells was measured by MTT assay at 24 and 48 h after treatment, respectively. One-way analysis of variance was used to analyze the results. Expression of CK 19 in BECs was monitored by flow cytometry and Western blot.RESULTS： Six lines of BECs were established. They survived for 4-18 wk in vitro. Flow cytometry analysis showed that these cells always expressed CK19. CsA, FK-506, RAD, and MMF inhibited proliferation of BECs in a dose-dependent manner. The lowest concentration of CsA, FK-506, RAD, and MMF to inhibit proliferation of BECs （P〈0.05） was 500, 100, 0.25, and 100 pg/L, respectively. However, the expression of CK19 by BECs was not changed.CONCLUSION： CsA, FK-506, RAD, and MMF have an antiproliferative effect on human intrahepatic BECs in vitro, while RAD has the strongest growth-inhibitory effect. Their possible effects on liver regeneration and bile duct injury in transplant patients should be further investigated.
瞄准：调查 cyclosporine 的效果 A (CsA )(MMF ) ， FK-506，和 mycophenolate mofetil 和 40-0-[2-hydroxyethyl ] 人的肝内的增长上的 rapamycin (RAD ) 胆汁的上皮细胞(BEC ) 在试管内。方法：BEC 与免疫从六个人的肝织物标本被孤立磁性的分离方法并且与 CsA， FK-506， RAD，和 MMF 在试管内的不同集中对待。房间的增长被 MTT 试金分别地在 24 和 48 h 术后疗法测量。变化的单程的分析被用来分析结果。在 BEC 的 CK 19 的表示被流动血细胞计数和西方的污点监视。结果：BEC 的六根线被建立。他们为 4-18 wk 在试管内幸存。流动血细胞计数分析证明这些房间总是表示了 CK19。CsA， FK-506， RAD，和 MMF 以一种剂量依赖者方式禁止了 BEC 的增长。禁止 BEC (P<0.05 ) 的增长的 CsA， FK-506， RAD，和 MMF 的最低集中分别地是 500， 100， 0.25，和 100 mug/L。然而，由 BEC 的 CK19 的表示没被改变。结论：CsA， FK-506， RAD，和 MMF 在人的肝内 BEC 在试管内上有 antiproliferative 效果，当 RAD 有最强壮的生长禁止的效果时。他们在 transplant 病人的肝新生和胆汁管损害上的可能的效果应该进一步被调查。
