摘要
以不同等电点的明胶为原料,用浊度分光光度法和毛细管等电点聚焦法测定了它们的等电点,用乳化法在归一化的介质酸碱度下制备了一系列微球,考察了明胶等电点对所制得微球的分散度的影响。结果表明,等电点越接近溶液pH值的明胶,制得的微球直径越小,分布越均匀,球形和流动性越好;等电点偏离溶液pH值越远的明胶,制得的微球直径越大,分布越不均匀,球形和流动性越差。因此,乳化法制备明胶微球时应该把溶液pH值调至尽可能接近所用明胶的等电点值。这是微球设计和制备必须遵循的一条重要准则。
Gelatins with different isoelectric points （IEP） were used as raw materials. Their IEP were determined with turbidimetry/speetrometry and eapillary IEP focusing methods. A series of gelatin mierospheres （GMs） were prepared with an emulsification method in a normalized acidity eondition. The influence of gelatins＇ IEP on the dispersivity of the GMs was examined. According to the experimental results, the GSIs prepared by a gelatin with its 1EP nearest to the medium pH have the smallest sizes, the narrowest size distribution, the best spherieity and fluidity; the farther the gelatin＇s IEP away from the medium pH, the larger the sizes, the wider the size distribution, the worse their sphericity and fluidity. In conclusion, the medium pH should be adjusted to approach to the IEP of the gelatin being used while preparing GMs by the emulsification method. It is a basic law governing the design and manufacture of the GMs.
出处
《明胶科学与技术》
CAS
2005年第4期203-210,共8页
The Science and Technology of Gelatin
