摘要
目的探讨细菌生物膜对留置尿管相关性尿路感染的影响。方法对留置尿管不同时间的43例患者,于撤除尿管时行尿液细菌培养,并采用阿利新蓝刚果红联合染色和扫描电镜观察尿管细菌生物膜形成情况,分析尿管细菌生物膜形成与尿管细菌培养及抗生素应用情况的关系。结果留置尿管患者尿管细菌生物膜阳性率,3d组为0(0/4),1周组33.3%(4/12),2周组71.4%(10/14),4周组100.0%(13/13)。应用抗生素组60.6%(20/33),未用抗生素组70.0%(7/10),2组差异无统计学意义,P>0.05。结论细菌生物膜形成是留置尿管相关性尿路感染的重要致病因素,抗生素预防治疗效果不明显。缩短尿管留置时间,采用封闭式引流等仍是尿路感染主要的防治方法。
Objective To explore the effect of bacterial biofilm on urinary tract infection associated with indwelling catheters. Methods This study included 43 patients who were catheterized for urine retention caused by BPH （ n =31 ） , urinary tract stricture （ n = 4 ） or urine drainage after operation （ n = 8 ）. The catheters were removed at 3d （n =4）, 7d （n = 12） ,14 d （n = 14） and 28d （n =13）, respectively, after they were stained with Alcian blue-Congo red; and the formation of bacterial biofilm in the catheters was observed under optical microscope, followed by examination of scanning electron microscopy and urine culture. Results There was no biofilm formation in catheters dwelling for 3 d in all the 43 cases. The biofilm positive rates of catheters dwelling for 7d, 14d and 28d were 33.3% （4/12） ,71.4% （10/14） and 100.0% （13/13）, respectively. The biofilm positive rate of catheters with antibiotic treatment was 60.6% （20/33）, while that with no antibiotic treatment was 70.0% （7/10）. There was no significant difference between the 2 treatment groups （P 〉0.05）. Conclusions Bacterial biofilm formation on the surface of the catheter is an important pathogenetic factor,which contributes to the recurrence and antibiotic resistance of urinary tract infection. Shortening the period of catheter dwelling and using closed drainage remain the predominant prevention and treatment of urinary tract infection.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2006年第2期135-138,共4页
Chinese Journal of Urology
