摘要
利用石英晶体微天平(QCM)实时监测低密度脂蛋白(LDL)在亲和素(Avid in)上的吸附,并探讨二者的作用机理。实验结果表明:Avid in与LDL之间的相互作用方式主要是静电吸附,即中性环境下带正电的Avid in可与LDL分子表面的带负电的磷脂分子发生静电吸引,当LDL浓度为0.049 5 g/L时,最大吸附量达到3.54×10-7g/cm2,吸附率为59.68%,NaC l溶液的洗脱效果达到了86%。研究证实:Avid in具有较大的吸附量和较高的吸附率,有望作为新型吸附LDL的吸附剂配基。
The use of quartz crystal microbalance （QCM） for monitoring in situ adsorption of low density lipoprotein （LDL） onto Avidin is described, and interaction mechanism is also discussed. Experimental results show that the binding of Avidin and LDL is electrostatic adsorption, because phospholipid with negatively charged at the surface of LDL had been found to mediate the interaction between LDL and Avidin under neutral condition. The maximal LDL adsorption capacity attains 3.54 × 10^ -7 g/cm^2 and the adsorptive efficiency calculated 59.68 % when LDL concentration is 0. 049 5 g/L. NaCl solution elution effect attains 86 %. In a word, a new method to evaluate the adsorption behavior is provided and Avidin is seen to be a potential lagand for LDL sorbent.
出处
《传感器技术》
CSCD
北大核心
2005年第12期46-48,共3页
Journal of Transducer Technology
基金
国家"863"计划资助项目(2002AA326060)
国家自然科学基金资助项目(90209054)
关键词
石英晶体微天平
低密度脂蛋白
亲和素
吸附
quartz crystal microbalance （ QCM ）
low density lipoprotein （LDL）
Avidin
adsorption
