摘要
我们提取纯化得到一种重均分子量为2.03×105、单糖为葡萄糖的香菇多糖级分(L-2)后,评价了香菇多糖L-2对荷S-180小鼠细胞免疫的影响。连续10d通过腹腔注射给予小鼠不同剂量的香菇多糖L-2(10mg/kg体重),测定了正常小鼠与荷S-180小鼠的血清中肿瘤坏死因子(TNF-α)和白介素2(IL-2)的含量,以及香菇多糖L-2对TNF-α和IL-2的mRNA表达的影响。与对照组小鼠相比,血清中TNF-α含量有显著增加,而血清中IL-2含量没有变化,与此同时,香菇多糖L-2可以上调TNF-α的mRNA的表达。结果揭示多糖L-2有增加TNF-α含量的作用,多糖增强免疫力与TNF-α含量增加以及在转录水平上调TNF-αmRNA的表达有关。
The effects of the polysaccharide （L-2） extracted from Lentinus edodes on the immune response of Sarcoma 180- bearing mice were evaluated, Mice were treated with two doses of polysaccharide L-2 （1, 10mg/kg body weight） for 10 days. The concentration of tumor necrosis factor-alpha （TNF- α ）, interferon-2 （IL-2） in mice serum and TNF- α mRNA expression were determined. The concentration of TNF- α in serum increased significantly in two doses groups compared to the model control group, but IL-2 not. The level of TNF- α mRNA transcription increased significantly in two doses groups to the model control group. Results of these studies demonstrated the polysaccharide L-2 significantly promoted TNF-α production, immunity potentiating and anti-tumor effects of the polysaccharide L-2 were associated with its potentiation of TNF-α mRNA expression at the transcriptional level.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第1期223-226,共4页
Food Science
基金
湖北省"十五"科技重点攻关项目(2001AA204A03)
关键词
香菇
多糖
肿瘤坏死因子
白介素2
Lentinus edodes
polysaccharide
tumor necrosis factor-alpha
interferon-2
