摘要
以甘肃漳县沙棘叶总黄酮含量为指标,采用正交试验对总黄酮的提取工艺进行研究。结果表明:最佳工艺参数为乙醇浓度70%,提取温度80℃,料液比1∶14,提取时间为2 h,该工艺条件可使黄酮提取率达97%以上。同时还比较了乙酸乙酯和水饱和正丁醇两种萃取溶剂。以水饱和正丁醇为萃取溶剂为佳,萃取率为70%以上,含量为5.6%。
On the index of content of total flavones in leaf of Hippophae rhamnoides L. from Zhangxian county of Gansu, the optimum extracting technology was investigated by using the orthogonal experiment. The result showed that 1：14 of 70% ethanol to leaf weight of Hippophae rharnnoides L. 2 h at 80℃ for each time were optimal parameter. Using this technology, the extraction rate of total flavones in leaf of Hippophae rharnnoides L. was over 97%. Meanwhile, ester extracts and n-butyl alcohol extracts were compared and the latter was a better method. Using the extracting solvent, the extraction rate was 70.4% and the content of total flavones was 5.6%.
出处
《西北农业学报》
CAS
CSCD
北大核心
2006年第1期148-151,共4页
Acta Agriculturae Boreali-occidentalis Sinica
关键词
沙棘
总黄酮
正交试验
Hippophae rhamnoides L.
Total flavones
Orthogonal experiment
