摘要
隐孢子虫病是一种重要的人畜共患原虫病。在家畜中以对反刍动物—奶牛的危害最大。当前,奶牛隐孢子虫主要还是以粪便检测为主,如改良抗酸染色法、金胺—酚改良抗酸复染法等。现在还有应用日趋广泛的免疫学检测方法,如ELISA、IFA、M cAb等;分子生物学检测方法,如常规PCR、巢式PCR等。本文对上述各种检测方法作一综述,以供广大寄生虫病学和兽医工作者参考。
Cryptosporidiosis is a important protozonic zoonosis. In livestock, it harms ruminant-dairy catties badly. Now, detecting Cryptosporidium oocysts in dairy cattle is mainly fccai-testing, including classical methods-Modified acid-fast staining, Auramine-phenol staining, immunological methods-ELISA, IFA, McAb, and molecular biological mothods-PCR, Nested PCR and so on. So this article with sum up different kinds of means for detecting. All of these will be supplied as references for personnel of researching parasites.
出处
《中国兽医寄生虫病》
2006年第1期32-38,共7页
Chinese Journal of Veterinary Parasitology
基金
国家科技部基础工作专项
畜禽重要寄生虫虫种资源的收集与保存(编号:2002DEB10050)
上海科技兴农重点攻关项目(编号:2004-12-3)
关键词
隐孢子虫病
检测
粪便
奶牛
Cryptosporidiosis
detection
fecal-testing
