摘要
目的观察银翘散对多种呼吸道病毒导致细胞病变的抑制作用,进一步探讨银翘散抗病毒机理。方法分别接种鼻病毒R_(14)型、流感病毒A型FM1株、呼吸道合胞病毒、腺病毒7型于KMB17、MDCK、Hep-2、Hela细胞,观察银翘散体外对多种呼吸道病毒作用。结果在4种培养细胞上,银翘散对4种呼吸道病毒株均未见明显的抑制病毒致细胞病变的作用。结论银翘散在体外对多种呼吸道病毒的增殖未显示抑制作用,结合体内抗病毒有效的结果,认为将来银翘散抗病毒机理研究方向可调整为以含药血清进行研究,模拟药物代谢的体内过程,使结果更符合临床特点,也可考虑银翘散可能通过对宿主防御机能的影响而发挥抗病毒感染效果。
Objective To observe the eytopathogenie inhibitory effect of Lonicerae and Forsythiae Powder on several respiroviruses and explore the mechanism of Lonicerae and Forsythiae Powder against viruses. Methods Rhinovirus type Rt4,Influenza vires type A FM 1 strain, RS virus, AD virus type 7 were inoculated into KMB17, MDCK, Hep - 2, Hela cells to observe the action of Lonieerae and Forsythiae Powder on several respiroviruses. Results No significant cytopatbogenic effect of Lonicerae and Forsythiae Powder inhibiting four respiroviruses on four kinds of cultured cells was observed. Conclusion Lonicerae and Forsythiae Powder does not have the inhibitory effect on generation of several respiroviruses in vitro. It is considered that researches on the of mechanism of Lonicerae and Forsythiae Powder against virus in the future should be directed at the following two aspects： （ 1 ） if serum containing drug was used to simulate hysiologieal disposition of drug metabolism the the results would be more consistent with clinical feature; （2） the anti - repiroviruses effect of Lonicerae and Forsythiae Powder maybe due to it action on the defense function of the host.
出处
《中国热带医学》
CAS
2006年第1期16-17,共2页
China Tropical Medicine
关键词
银翘散
体外
呼吸道病毒
研究方向
Lonieerae and Forsythiae Powder
in vitro
Respiroviruses
