摘要
目的:探究终末期肾功能衰竭患者T细胞亚群的凋亡受体CD95分子与共刺激分子CD28、CD152(CTLA-4)的表达与细胞免疫功能的关系。方法:采用流式细胞术检测外周血T细胞的凋亡受体CD95(Fas)与共刺激分子CD28/CD152表达。结果:终末期肾功能衰竭患者CD3+T细胞和CD4+T细胞比例明显高于健康对照组,CD4/CD8比值增加(P=0．008), CD4+T细胞和CD8+T细胞上CD95分子表达均上调(P=0．001),以CD8+T细胞上CD95分子增加更为明显;CD28和CD152 分子在不同T细胞亚群上的表达均上调(P<0．05),然而,CD4+T细胞以CD28分子表达增加为主,而CD8+T细胞则CD152 分子表达增加为主。结论:终末期肾功能衰竭患者的细胞亚群失衡,共刺激分子CD28和CD152表达异常增加,提示T细胞活化与抑制性调节发生紊乱。T细胞亚群上凋亡受体CD95分子表达增加,以CD8+T细胞为主,说明终末期肾功能衰竭者淋巴细胞的减少可能通过两种途径——受体配体途径和负性共刺激分子CD152抑制信号途径,其中以CD8+T细胞减少为主,造成患者细胞免疫缺陷。
Objective ：To explore the relationship of expressions of apoptotic receptor CD95 and costimulators of CD28/CD152 （CTLA-4） on T lymphocytic subpopulafions with cellular immune in patients with end-stage renal failure（ESRF）. Methods;To apply flow cytometry to determine expressions of CID5 and CD28/CD152（CTLA-4） on T cell subsets in patients with ESRF. Results：In comparison between patients group and control group,CD3^＋T cells and CD4^＋T ceils strikingly increase in patients,the ratio of CD4^＋ and CD8 is increasing（ P=0. 008）;expressions of CD95 on both of CD4^＋ and CD8^＋T cells are increasing（ P = 0. 001 ） ,however, CD95 is expressed more dominantly on CD8^＋T cells than on CD4^＋ T cells;expressions of costimulators on T cell subsets are increasing （P 〈 0. 05 ） ,in which CD4^＋ CD28^＋ T cells and CD8^＋CD152^＋ T cells are dominant. Conclusion： Imbalanced T lymphocytic subpopulations and the abnormal expressions of CD28/CD152 in patients with ESRF suggest that activated and suppressed regulations of T cells are disturbed ,and expressions of CD95 are more dominant on CD8^＋ T cells ,which indicates lymphocytes decrease in patients possibly mainly by two pathways death receptor-ligand pathway and the suppressed signal induced by negative cosfimulator CD152, so that the striking reduce of CD8^＋ T cells leads to the defect of cellular immune in patients.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第1期85-88,共4页
Chinese Journal of Immunology
