摘要
目的观察哌拉西林/三唑巴坦对临床常见致病菌的体外抑菌活性,比较国产与进口哌拉西林/三唑巴坦的体外抑菌效果。方法将336株实验菌株分别制成菌液,质控菌株为大肠埃希菌25922和铜绿假单胞菌27853。采用琼脂二倍稀释法测定各种药物的体外抑菌效果,并根据抗生素药物敏感的临界浓度计算细菌对药物的敏感率。结果国产哌拉西林/三唑巴坦对肺炎克雷白菌[超广谱β-内酰胺酶阳性(ESBLs+)]、肺炎克雷白菌(ESBLs-)、大肠埃希菌(ESBLs+)、大肠埃希菌(ESBLs-)有良好的抑菌作用,对铜绿假单胞菌的抑菌效果亦较好。与头孢哌酮/舒巴坦、头孢噻肟/舒巴坦、阿莫西林/克拉维酸钾和氨苄西林/舒巴坦等药物相比,哌拉西林/三唑巴坦体外抑菌效果最好。肠杆菌科细菌对国产与进口哌拉西林/三唑巴坦的敏感率几乎无差异,国产与进口哌拉西林/三唑巴坦对铜绿假单胞菌的抑菌效果相当。结论哌拉西林/三唑巴坦具有较强的抑菌作用,国产与进口哌拉西林/三唑巴坦的体外抑菌效果相当。
Objective To survey and compare the in vitro bacteriostatic effects of domestic and imported piperacillin/ tazobactam on Pathogenic bacteria commonly encountered in the clinic. Methods Bacterial suspensions were separately prepared for each of the 336 strains of bacteria to be tested. Escherichia coli 25922 and Pseudomonas aeruginosa 27853 surved as the quality control bacteria strains. The in vitro bacteriostatic effects of various drugs were assayed with the doubled agar dilution method. Sensitivity of bacteria to drugs was calculated according to the marginal antibiotics＇ concentration to which the bacteria were sensitive. Results Domestic piperacillin/tazobactam was shown to have excellent baeteriostatie effects on Klebsiella pneumoniae I super broad spectrum β-lactamase positive （ ESBLs ＋ ）, K. pneumoniae（ ESBLs-）, E. coli（ ESBLs ＋ ）, E. coli （ESBLs-） and a fairly good inhibitory effect on P. aeruginosa. Piperacillin/tazobactam was also shown to have the best in vitro bacteriostatic effects as compared with those of cefoperazone/sulbactam （ CFP/SUB ）, cefotaxime/sulbactam （ CTX/SUB ）, amoxicillin/clavulaniate potassium （ AMO/CLK ）, and ampicillin/sulbactam （ AMP/SUB ）. Besides, the enterobacteriaceae manifested almost the same sensitivity to domestic and imported piperacillin/tazobactam and the 2 kinds of the drug showed equivalent bacteriostatic effects on P. aerllginosa. Conclusion Piperacillin/tazobactam was shown to have excellent in vitro bacteriostatic effects and domestic and imported products of the drug were comparable in this respect.
出处
《医药导报》
CAS
2006年第2期156-158,共3页
Herald of Medicine
关键词
哌拉西林/三唑巴坦
抑菌作用
体外
最小抑菌浓度
Piperacillin/tazobactam
Bacteriostatic effect, in vitro
Minimal inhibitory concentration （MIC）
