摘要
通过胶体金标记提纯后的兔抗ＩＢＶＩｇＧ，建立了一种以微孔滤膜为固相载体，以红色胶体金为标记物，检测鸡传染性支气管炎病毒（ＩＢＶ）的斑点免疫金测定法（ＤＩＧＦＡ）。ＤＩＧＦＡ对ＩＢＶ的最小检出量为２０．３×１０￣（－８）ｇ，在１０份自然病例样品中检出８份。鸡胚培养法以育传３～５代，出现侏儒胚者为阳性，从上述样品中检出６份。对接种ＩＢＶ的鸡胚尿囊液，ＤＩＧＦＡ法检出率为１００％。实验结果表明，ＤＩＧＦＡ法对鸡传染性支气管炎的早期诊断具有简便、灵敏、快速、特异性强和重复性好等优点。
The purified rabbit anti-IBV IgG was labelled with colloidal gold.DIGFA withamicrofilter paper as carrier and the red colloidal gold as label was developed for detectionof avian infectious bronchitis virus(IBV).The minimum amount of IBV giving a positivereaction (1 +,Red dot)was 20.3 × 10(-8)·g(-1). Comparing the DIGFA with virus embryoculture assys,8 of l0 samples (80%)in naturally infected chicks were positive with DIGFAbut 6 of l0 were positive(60%)with virus embryo culture assay. The positive rate for al-lantoic fluid of experimentally inoculated embryonated eggs was l00%with DIGFA. Theresults indicated that the DIGFA had the advantages of simplicity,high sensitivity,strong,specificity, rapidity and good repetitiveness for early diagnosis of infectious bronchitis inchicken.
出处
《河南农业大学学报》
CAS
CSCD
1996年第2期116-119,共4页
Journal of Henan Agricultural University
关键词
斑点免疫
传染性支气管炎
病毒
检测
鸡病
dot Immuno-gold fitration assay(DIGFA )
infectious nronchitis nirus(IBV)
detection
