摘要
本文建立了用高效液相色谱法（ＨＰＬＣ）分离人红细胞膜脂肪酸的方法。选用２－溴苯乙酮为衍生剂、１８－冠醚－６为催化剂，有Ｇ＿（１８）反相柱分离，流动相采用甲醇－乙腈－水，正十六酸（Ｃ＿（１７）：０）为内标，紫外测定波长为２５４ｎｍ，于６０ｍｉｎ内分离了人红细胞膜含有的八种主要脂肪酸。
high-performance liquid chromatographic method was established for examining fatty acids in human ery-throcyte membrane.The fatty acids were derivatized with 2-bromoacetophenone The reaction was catalyzed by18-Crown-6.The fatty acid esters wer analysed on a C18 reversed-phase‘column with methanol-acetonitrile-water as a mobile phase and heptadecanoic acid(17:0)as the internal standard.The compounds、vere detected at 254mm.The eight kinds of fatty acids in human erythrocyte membrane were separated within 60 min.
出处
《重庆医科大学学报》
CAS
CSCD
1996年第1期10-12,共3页
Journal of Chongqing Medical University
