摘要
目的探讨炎症细胞与地塞米松对基质金属蛋白酶活性的影响及基质金属蛋白酶在肺纤维化中的作用。方法①实验于2004-04/07在解放军兰州军区总医院动物实验中心和呼吸内科实验室、血液科实验室完成。选用32只普通W istar大鼠。随机将动物分为3组对照组(n=8),肺纤维化组(n=12),治疗组(n=12)。②肺纤维化组和治疗组按5m g/kg经气管内灌注博莱霉素溶液0.3m L制备大鼠肺纤维化模型,对照组则给予0.3m L生理盐水,治疗组每日腹腔注射地塞米松0.4m L(5m g/kg),对照组及肺纤维化组则每日腹腔注射0.4mL生理盐水。③在实验第3,7,14,27天分别处死后,收集肺泡灌洗液,行细胞计数和分类;聚丙烯酰胺明胶酶谱法鉴定肺泡灌洗液中基质金属蛋白的活性变化;羟脯氨酸法测定肺组织胶原含量的变化。结果大鼠32只均进入结果分析。①病理组织切片示肺纤维化组肺损伤明显,出现典型肺纤维化改变;治疗组肺损伤和纤维化程度明显减轻。②治疗组肺泡灌洗液粒细胞学分类在第7,14天分别比肺纤维化组降低了51.6%和62.4%(P<0.05);第14天治疗组肺胶原总量比肺纤维化组降低了17.8%(P<0.05)。③聚丙烯酰胺明胶酶谱示基质金属蛋白酶2和基质金属蛋白酶9在两组中活性带均增强,但治疗组活性带的强度比肺纤维化组明显减弱。结论炎症细胞能增强基质金属蛋白酶的活性,基质金属蛋白酶活性增强进一步促进了纤维化的发展,而地塞米松则抑制了基质金属蛋白酶活性增强,减轻了肺纤维化程度。
AIM： To investigate the effects of inflammatory cells and dexamethasone on the activity of matrix metalloproteinases and the role of matrix metalloproteinase in pulmonary fibrosis. METHODS： （1） The experiment of carried out in the animal experimental center and labs of the Department of Respiratory Medicine and Department of Hematology in Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA hetween April and July 2004. Thirty-two common Wistar rats were randomly divided into control group （n =8）, pulmonary fibrosis group （n=12） and treatment group （n=12）. （2） The rats in the pulmonary fibrosis group and treatment group were made into models of pulmonary fibrosis by perfusing 0.3 mL bleomycin solution （5 mg/kg） via trachea, but those in the control group were given 0.3 mL physiological saline. The rats in the treatment group were treated with daily intraperitoneal injection of 0.4 mL dexamethasone （5 mg/kg）, and those in the control group and pulmonary fibrosis group were treated with daily intraperitoneal injection of physiological saline. （3） The rats were killed at 3, 7, 14 and 27 days, and the bronchoalveolar lavage fluid of the rats was harvested, and then the cells were counted and sorted. The changes of the activities of matrix metalloproteinases in bronchoalveolar lavage fluid were detected with gelatin zymography, and the changes of collagen contents in lung tissue were determined with hydroxyproline assay. RESULTS： All the 32 rats were involved in the analysis of results. （1） The pathohistological section demonstrated that the lung injury was obvious in the pulmonary fibrosis group, and typical changes of pulmonary fibrosis were observed, and the severities of lung injury and ulmonary fibrosis in the treatment group were obviously relieved. （2） The granulocytic classifications of bronchoalveolar lavage fluid at 7 and 14 days in the treatment group were decreased hy 51.6% and 62.4% as compared with those in the pulmonary fibrosis group （P
出处
《中国临床康复》
CSCD
北大核心
2005年第47期66-68,共3页
Chinese Journal of Clinical Rehabilitation
基金
兰州军区医药卫生科研基金资助项目(LXH02-09)~~
