摘要
目的探讨葡萄糖激酶（GCK）基因及细胞毒性T淋巴细胞抗原（CTLA-4）基因与妊娠糖尿病（GDM）的关系。方法采用1：4配比病例对照研究方法，对进行孕期健康检查的孕妇以问卷调查的方式收集一般资料，并采集被调查者血样。采用聚合酶链反应-单链构象多态性分析（PCR-SSCP）和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测GCK基因和CTLA-4基因突变情况。结果CTLA-4外显子1第49位核苷酸A／G变异和CTLA-4启动子-38位核甘酸C／T变异与GDM有统计学联系，其OR及95％CI分别为4，510（1．810～11．238）和8．918（1．558～51．040）。未发现GCK基因外显子6和外显子7突变与GDM的发生之间有这联系。结论CTLA-4外显子1等基因A／G变异、CTLA-4YNK FCL BB-38位核苷酸C／T变异是GDM发生的危险因素。
Objective To explore the associations between glucokinase （GCK） gene, cytotoxic T cell antigen - 4 （CTLA- 4） gene and gestational diabetes mellitus （GDM）. Methods A hospital-based 1 ： 4 matched case-control study was conducted. Each GDM case was matched with four controls on age. 21 GDM cases and 84 pregnant women without GDM were recruited. The mutations at exon6 and exon7 of GCK gene were determined by PCR-single-strand conformation polymorphism analysis. The mutations at exonl and promoter of CTLA- 4 gene were determined by PCR-restriction fragment length polymorphism analysis. A questionnaire was used to get the information about demographic characteristics, individuals and families. Results Results form conditional logistic regression analysis showed that the A-G transition polymorphism at 49 position （exonl, codonl 7） （OR = 4.510 95% CI 1. 810- 11.238） and the C-T transition polymorphism at position - 318 （promoter） of CTLA4 gene （OR = 8.918 95% （CI 1.558 - 51.040） was associated with the developent of GDM. The association between GCK gene and the develoment of GDM was not found. Conclusion The A-G transition polymorphism at exonl and the C-T transition polymorphism at promoter of the CTLA4 gene were associated with higher risk for the development of GDM.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第1期3-4,共2页
Chinese Journal of Public Health
基金
国家自然科学基金项目(33970661)
