摘要
无论浸种还是叶面喷施,与单独使用DPC相比,DPC与DTA6复配在促进转基因抗虫棉幼苗根长和侧根原基增加的同时,显著减弱了DPC对地上部生长的延缓作用,中棉所30下胚轴长度较对照仅缩短3%左右,中棉所30、新棉99B和sGK321的苗期叶面积均相当于对照的93%～94%,这可能与复配调节剂处理后转基因抗虫棉幼苗各部位的IAA浓度较DPC单独处理明显提高有关。DPC与DTA6复配还可显著增加转基因抗虫棉苗期功能叶叶绿体的基粒类囊体垛叠程度,提高光合作用。
Most insect-resistant transgenic cotton developed slowly in North China Plain, which made it susceptible to plant growth retardant DPC. The objective of this research was to verify the effects of mixture of DPC and plant growth accelerant DTA-6 （diethyl aminoethyl hexanoate） on seedling development of insect-resistant transgenic cotton and to offer data for developing new plant growth regulator fitting to it. Compared with single application of DPC, the mixture of DPC and DTA-6 not only increased the root length and the number of lateral root primordia of insect-resistant transgenic cotton, but also significantly diminished the inhibition of up-ground part caused by DPC. For example, the hypocotyls length of CCRI 30 decreased about 3% only, and the leaf area of seedlings of CCRI 30, DP99B and sGK321 was all equivalent to 93%-94% of respective control. These results were attributed to the increase of IAA concentration, in top, cotyledon and hypocotyls of insect-resistant transgenic cotton. Furthermore, the mixture of DPC and DTA-6 increased the number of granalamellae in chloroplast and enhanced the photosynthetic rate of functional leaf of insect-resistant transgenic cotton. Obviously, the mixture of DPC and DTA-6 retained the function of enhancing root development of DPC, which was in favor of adversity-resistance and preventing premature senescence. At the same time, DTA-6 counteracted the inhibition of up-ground part caused by DPC in great extent, which was in favor of seedling rapid growth of insect-resistant transgenic cotton.
出处
《棉花学报》
CSCD
北大核心
2006年第1期3-7,共5页
Cotton Science
基金
国家863计划(2001AA246062
2003AA246060)
农业结构调整重大专项(040701A)
天津市与中国农大合作项目(04030)
关键词
DPC
DTA-6
复配
抗虫棉
生长发育
DPC
DTA-6
mixture^insect-resistant transgenic cotton
growth and development
