摘要
目的探讨体内药敏实验中化疗前后瘤细胞凋亡指数/增殖指数变化的意义.方法①裸鼠60只分为生理盐水组及5-Fu、DDP、MMC化疗组,每组15只.所有小鼠均采用同一新鲜胃癌标本进行SRCA实验.测量移植瘤体积变化差值△TS值,计算肿瘤消退率RR(%).②分别用免疫组化和TUNEL方法检测移植瘤及肿瘤标本的增殖指数和凋亡指数.③比较分析移植前后瘤的在肿瘤消退率、凋亡指数与增殖指数的差异.结果6天法SRCA中,敏感组移植前后瘤细胞凋亡指数/移植瘤的增殖指数(AI/PI)的差异有显著性,两不敏感组中移植前后瘤细胞的(AI/PI)的差异无显著性(p>0.05).结论在裸鼠SRCA药物敏感性试验中,瘤细胞移植前后AI/PI的变化对评价体内药物敏感性有重要意义.
Objective To investigate the changes of AI/PI of the xenogrefts in vivo. Methods The nude mice（n=60） which were subdivided into four subgroups respectively. SRCA were carried out with fresh explantation of the gastric carcinoma in two groups, the mice were treated with MMC、5-Fu、DDP and 0.9% salt solution respectively. All mice were killed and the change of tumor size （△TS） was observed after SRCA. The tumor debulking rate （RR） was calculated. The PI of gastric carcinoma and xenografts were detected with immunohistochemical method, and the AI of gastric carcinoma and xenografts were detected with TUNEL assay. Compared and analyzed the changes of the AI/PI with SPSS 11.0. Results According to the T-test, there was no statistical difference of the changes M/PI in the NS and MMC groups （p〉0.05）, but there were high statistical difference in between 5-FU and DDP groups. Conclusions In the SRCA, the changes of AI/PI of the tumor may be useful for Chemosensitivity.
出处
《现代临床医学生物工程学杂志》
2005年第6期487-489,共3页
Journal of Modern Clinical Medical Bioengineering
