摘要
本研究建立了变形链球菌ＭＴ８１４８转化系统。在本组转化培养条件下，变形链球菌ＭＴ８１４８在对数生长早期出现感受态但维持时间很短，供体ＤＮＡ最适浓度为２５μｇ／ｍｌ，超螺旋闭环质粒与线性质粒均可转化变链ＭＴ８１４８。与变链ＧＳ５相比。
Streptococcus mutans MT8148 (serotype C) was transformed to erythromycine or kanamycine resistance with a E. coli-Streptococcus shutter vector pVA838, pZβ1, a pVA838 derivative containing gtfBgene from S.mutans MT8148, a suicide plasmid pTF55 containing the inactivated gftB gene and the linealized pTF55 by KpnI. The maximal transformation was achieved by incubating the recipient cell for 1.5 hours and adding donor DNA concentration to 25μg/ml.
出处
《牙体牙髓牙周病学杂志》
CAS
1996年第1期23-25,共3页
Chinese Journal of Conservative Dentistry
