摘要
目的:从真菌松萝中提取地衣酸并研究地衣酸对体外培养前列腺癌PC-3M细胞增殖的抑制效应.方法:用细胞培养、细胞生长抑制实验(MTT法)观察PC-3M细胞形态、细胞生长密度和检测细胞生长抑制率,用3H-TdR掺入法测定细胞增殖过程中DNA的含量.结果:与对照组相比,实验组PC-3M细胞形态异常、细胞生长密度降低,细胞生长抑制率增强,3H-TdR掺人量明显减少,并呈剂量依赖关系,与地衣酸浓度的相关系数分别为0.9799与-0.9525.结论:地衣酸对体外培养PC-3M细胞具有抑制效应.
Objective： To extract Usnic acid from usnea longisima ach, and to evaluate the ability of Usnic acid to inhibit growth of human prostate cancer cells PC-3M in vitro. Methods： The cells were observed by microscope. The cytotoxicity to cancer cells was determined by MTT assay. DNA synthesis was tested by 3H-TdR in corporation assay in the experiments. Results： There were significant differences between PC-3M cells lest group and control group. The Usnic acid had strengthened cytotoxicity, and the rate of 3H-TdR incorporation was lower than that of the control in a dose-dependent manner （ r = 0. 9799, r = - 0. 9525 ）. Conclusion ： The results suggested that the Usnic acid has inhibitory effects on growth of human PC-3M cells in vitro.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2005年第4期289-291,共3页
Chinese Journal of Cancer Biotherapy
基金
日本国际协力事业团(JI-CA)资助专项技术合作项目第59项
