摘要
目的:研究血管内皮生长因子(VEGF)在乳腺癌组织中的表达及其与临床生物学特性的关系。方法:应用免疫组化S-P法和RT-PCR法检测VEGF在42例乳腺癌和相应正常乳腺组织中的表达。结果:免疫组化和RT-PCR检测结果显示,42例乳腺癌组织中VEGF的阳性表达率分别为59·5%(25/42)和73·8%(31/42),明显高于正常乳腺组织VEGF的阳性率16·7%(7/42)和23·8%(10/42),VEGF的高表达与乳腺癌患者淋巴结转移密切相关。结论:乳腺癌组织中存在VEGF的过量表达,并且与癌组织淋巴结转移有关。检测VEGF有可能作为预测乳腺癌转移潜能和预后的重要指标。
Objective：To investigate the relationship between expression of vascular endothelial growth factor （VEGF） and cancer biological characteristics in mammary carcinoma. Methods ： Expression of VEGF in 42 cases of mammary carcinoma and their adjacent normal breast tissues were detected by immunobistochemistry and reverse transcription polymerase chain reaction （RT-PCR） techniques. Results ： The positive rates of VEGF in 42 cases of mammary carcinoma were 59.5% （ 25/42 ） and 73.8% （ 31/42 ） detected by immunohistoehemistry and RT-PCR, respectively, which were higher than those in normal breast tissues of 16.7% （7/42） and 23.8% （10/42）. VEGF expression was closely related to tire metastasis of mammary carcinoma, Conclusion：VEGF overexpression existed in mammary carcinoma, and related to cancer metastasis. VEGF may be a target marker in forecasting tire metastatic ability and prognosis of mammary carcinomas.
出处
《临床肿瘤学杂志》
CAS
2005年第6期624-626,630,共4页
Chinese Clinical Oncology
关键词
乳腺肿瘤
血管内皮生长因子
转移
免疫组化
逆转录聚合酶链反应
Mammary carcinoma
Vascular endothelial growth factor
Metastasis
Immunohistoehemistry
Reverse transcription polymerase chain reaction
