摘要
目的利用核仁组成区技术(AgNOR)区分良性、中至重度不典型增生及恶性细胞,并探讨AgNOR分型与癌细胞类型的关系。方法应用胶银染色技术检测439例细胞学涂片。结果良性细胞均数(2.96±0.76)与恶性细胞均数(9.90±2.43)差异有极显著性(P<0.001),说明产生假阳性机会很小。良性细胞与中度不典型增生细胞均数(3.90±0.66)和重度不典型增生细胞均数(5.88±0.98)差异有极显著性(P<0.001),有助于识别可疑癌细胞。AgNOR在腺癌细胞中颗粒粗大、色深;在鳞癌及小细胞癌中颗粒多为弥散型,细小、色浅。AgNOR分型与癌细胞分型有显著相关性(P<0.001)。用AgNOR计算标准判断良恶性其敏感性为96.4%,特异性为95.5%。结论细胞学普通染色诊断困难时,AgNOR银染技术可以辅助诊断。
Objectives To differentiate cells of benign from those of moderate and severe atypical hyperplasia and malignant by AgNORs technique and to explore the relation between the types of cancer cells and AgNOR typing. Methods Ag staining was used in cytological diagnosis of 439 cases. Results Difference between mean values of benign group and malignant group was obvious （P 〈 0.001 ）. Rate of false positivity was low. Difference between mean values of benign cells, moderate and severe atypical hyperplasia cells and malignant cells was significant（ P 〈 0.001 ）, which was helpful to identifying suspicious cancer cells. AgNOR particles were bigger and deeper in color in adenocarcinoma than in squamous cancer cell and small cell cancers. There was obvious relation between types of AgNOR particles and those of cancer cells （ P 〈 0. 001 ）. AgNOR sensitivity and specificity in differentiation of benign and malignant cells were 96.4% and 95.5% respectively by AgNOR count criterion. Conclusions AgNOR technique can help diagnosis for which it is difficult to do by routine staining.
出处
《中国肿瘤临床与康复》
2005年第6期507-509,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
核仁组成区
细胞学
肿瘤细胞
Nuclear organizer region
Cytology
Tumor cell
