摘要
背景:溃疡性结肠炎(UC)与感染性结肠炎(IC)均为结肠性腹泻的常见原因,即使综合临床表现、内镜和组织学检查等表现,仍有部分UC与IC难以鉴别。目的:比较抗中性粒细胞胞浆抗体(ANCA)、CD44v3、CD44v6和转化生长因子(TGF)β1在UC和IC中表达的差异,为两组患者提供有用的鉴别指标。方法:采用间接免疫荧光法(HF)和酶联免疫吸附法(ELISA)检测血清ANCA和ANCA靶抗原;免疫组化法检测两组患者结肠黏膜CD44v3、CD44v6和TGFβ1的表达。结果:血清ANCA检测UC患者阳性率HF法为48.1％,ELISA法为33.3％,而IC组均为阴性,两组比较差异有显著性(P<0.05);结肠黏膜CD44v3、CD44v6在UC患者表达阳性率分别为68.8％、56.3％。而IC组均阴性,两组比较均有显著差异(P<0.05);两组患者结肠黏膜TGFβ1的表达无显著差异(P>0.05)。结论:血清ANCA和结肠黏膜CD44v3、CD44v6的检测有助于鉴别UC与IC。
Background： Ulcerative colitis （UC） and infectious colitis （IC） are common causes of colonic diarrhea. It＇s difficult to differentiate UC from IC clinically even through a combination of clinical manifestations, endoscopic and histological examinations. Aims： To elucidate the differences between UC and IC on expression of anti-neutrophilic cytoplasm antiboby （ANCA）, CD44v3, CD44v6 and TGFβ1, and to supply helpful indexes for differentiating UC and IC. Methods： Serum ANCA was detected by indirect immunofluorescence technique （IIF） and enzyme linked immunosorbent assay （ELISA）. Expression of CD44v3, CD44v6 and transforming growth factor （TGF）β1 on colonic mucosa were detected by immunohistochemistry. Results： The serum ANCA was found in 48.1% of UC patients by IIF, and 33.3% positive by ELISA. No expression of serum ANCA was found in IC patients. There was significant difference between the two groups （P〈0.05）. Expression of CD44v3 and CD44v6 on colonic mucosa was 68.8%, 56.3% in UC patients respectively, but no expression in IC patients, there were significant differences between the two groups （P〈0.05）, but no differences in TGFβ1 detection on colonic mucosa between the two groups （P〉0.05）. Conclusions： The detection of serum ANCA, CD44v3 and CD44v6 on the colonic mucosa surface may be helpful in differentiating UC from IC.
出处
《胃肠病学》
2005年第6期339-342,共4页
Chinese Journal of Gastroenterology
