摘要
将３２例肛管和肛周尖锐湿疣进行Ａｐｇａｒ评分和凹空细胞分型，应用聚合酶链反应和免疫组化技术检测ＨＰＶ抗原和ＤＮＡ，并观察ＨＰＶ在尖锐湿疣组织中的分布状况。结果发现Ａ型１４例，Ｂ型１８例，Ａ型与高积分尖锐湿疣的关系较Ｂ型密切。ＰＣＲ和免疫组化检测阳性率分别为２５％（８／３２）和２２％（７／３２）。我们发现在尖锐湿疣组织中，不论是否是凹空细胞或凹空细胞分型如何及Ａｐｇａｒ积分高低，均有ＨＰＶ分布，但阳性率不同。这表明在正确诊断尖锐湿疣时，凹空细胞核形态、组织学改变和特殊检测三者各起作用并彼此相关。本文还探讨了浆膜型抗原发生机制。
Thirty-two cases of anal and perianal condyloma acuminata(CA)were given a total Apgar wart score and assessed as type A or B according to their koilocyte nuclear morphology. We detected the presence of HPV 6 DNA and HPV antigen by polymerase chain reaction (PCR) and immunohistochemistry(IHC) techniques and also observed the HPV distribution in CA.14 cases of CA were type A and 18 type B.Type A koilocytes were associated with higher total wart scores than type B. From 32 cases, HPV 6 DNA sequences were amplified in 8 cases(25%)and HPV antigen present in 7 cases (22%). HPV present not only in type A koilocytes and high score CA, but also in type B ones and low score CA,even in non-koilocytes.But the HPV positive rates were different.We found the respective roles of nuclear morphology,histopathological features and HPV detection and their close correlation in the correct diagnosis of CA.The mechanism of the presence of cytoplasm-membrane associated HPV antigen was discussed.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
1996年第1期11-14,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
肛管
肛周
尖锐湿疣
聚合酶链反应
人乳头状瘤
anus
condylomata acuminata
immuohistochemistry
polymerase chain reaction
human papillomavirus
