摘要
３１只大白鼠随机分成５组，Ⅰ组为对照组。Ⅱ组静脉输注７．５％葡萄糖生理盐水；Ⅲ组２０％葡萄糖和３．５％氨基酸液（Ｍｏｌｉｐｒｏｎ）；Ⅳ组２０％葡萄糖和３．５％氨基酸液（Ａｍｉｎｉｃ）；Ⅴ组３０％葡萄糖和３．５％氨基酸液（Ａｍｉｎｉｃ）。本实验所用两种氨基酸液的主要不同在于支链氨基酸（ＢＣＡＡ）的含量不同。给各组大白鼠输注不同静脉营养液７天后，取肝组织行光学和电子显微镜的研究。Ⅰ、Ⅱ组组织学上未见明显异常。Ⅲ、Ⅳ、Ⅴ组皆出现肝细胞的脂肪浸润，但以Ⅴ组最严重，Ⅲ组次之，Ⅳ组最轻。后３组也皆出现了肝内胆汁淤积的病理改变，以Ⅲ组最严重，Ⅳ组与Ⅴ组的病理改变几乎相等。本实验结果显示：（１）静脉营养液中过高浓度的葡萄糖可引起肝脂肪变性。（２）某些氨基酸的缺乏或过量也可导致肝脂肪变性。（３）输注葡萄糖液体与禁食皆与胆汁淤积无关。（４）某些氨基酸的缺乏或某些氨基酸的直接毒性作用与胆汁淤积有关。（５）支链氨基酸对胆汁淤积有预防作用。
Abstract In this study, 31 rats were divided randomly into 5 groups. Group Ⅰ served as control; Group Ⅱreceived 7. 5%glucose salt solution; Group Ⅲ, TPN with 20%glucose and 3. 5% Molipron; Group Ⅳ, 20% glucose and 3. 5% Aminic; Group Ⅴ, 30% glucose and 3.5% Aminic. Seven days after liver cytology was studied by light and electron microscopy. There were no obvious pathological changes in group Ⅰ、Ⅱ. Fatty liver developed in group Ⅲ、Ⅳ、Ⅴ.The cholestatic signs were seen in group Ⅲ、Ⅳ、Ⅴ.Conclusions:(1) Fatty liver could be caused by disproportionate individual amino acids.(2)Glucose or starvation have no effect on cholestasis.(3) The aminoacid composition contributes to the TPN-cholestasis. (4) BCAA prevents TPN-cholestasis.
出处
《普外临床》
CSCD
1996年第1期39-40,共2页
