摘要
黑曲霉孢子经海藻酸钙包埋３３ｈ后得到的固定化增殖细胞其酶活性达到最高，机械强度仍保持较高水平（为起始时８０％），最适ｐＨ为５．０，与游离酶相同，最适温度为５５℃，比游离酶高５℃；有害离子对其影响较小，其Ｋｍ（以蔗糖为底物）为８２ｍｍｏｌ．将固定化增殖细胞装入填充式反应柱，产品中低聚果糖含量在５０％～５５％，操作一个月活性保持不变。
The spores of A. niger were immobilized for production of fructo-oligosaccharides.After incubation for 33 hours,the biocatalyst reached maxmum activity and mechanical stability decreased to 80% of the initial strength.The optimum pH and temperature of the immobilized growth cell were 5.0 and 55℃ respectively.The optimum PH was the same but the optimum temperature was higher by 5℃ compared with that of non-immobohjed enzyme.The immobilized beads were less sensitive to metal ions especially to Hg2+ than the non-immobohjed enzyme.The immobilized cells were packed in a column for continuous production of FOS and 50%-55% FOS(FOS/total sugar) was produced at the flow rate of lml/min. After continuous operation for more than one month,no loss of activity was observed.
关键词
低聚糖
固定化
低聚果糖
增殖细胞
黑曲霉
Oligosaccharides
Microorganism
Immobilization/Fructo-oligosaccharides
Growth cells
Aspergillus niger
