摘要
目的:建立人血浆中他扎司特的活性代谢物 MTCC 浓度的反相离子对高效液相色谱测定方法。方法:用外标法。血浆样品经酸化,用乙酸乙酯萃取后进样。色谱柱:YWG-C_(18)(4.6mm×250mm,10μm);流动相:乙腈-0.05 mol·L^(-1)磷酸盐缓冲液(1:4,pH=6.5),其中含0.004mol·L^(-1)四丁基溴化铵;流速:1.0 mL·min^(-1);紫外检测波长:254 nm。结果:血药浓度在0.02～2.00μg·mL^(-1)范围内呈良好的线性关系,r=0.9991,方法平均回收率为99.2%,日内 RSD=7.4%,日间 RSD=8.4%,平均萃取后收率为80.5%。结论:本方法操作简单,快速,灵敏度高,结果准确,重现性好,可用于他扎司特的药代动力学和生物利用度的测定。
objective：A RP - HPLC method was developed for the determintion of tazanolast＇s active metabolite MTCC in human plasma. Methods. The procedure involved acidulation of plasma and a single solvent extraction of drug from plasma into ethyl acetate. The column：YWG -C18 （4.6 mm 250 mm, 10 μm） ;The mobile phase：acetonitrile - 0. 05 mol · L^-1 phosphate buffer solution （ 1 ： 4, pH = 6. 5 ）, and 0. 004 mol · L^-1 tetrabutylammonium bromide in it. The flow rate：1. 0 mL · min^-1. The detective wave length ：254 nm. Results：The regression line was linear over the plasma concentration range of 0.02 -2. 00 μg · L^-1,r =0. 9991 ,the average methodological recovery of MTCC was 99. 2%, and the average extraction recovery was 80. 5% ,the RSD of within -day and between -day was 7.4% and 8.4% ,respectively. Conclusion： ：The method is simple, rapid, results is accurate and good reproducible. The analytic condition could be used to study the pharmacokineties and relative bioavailability of tazanolast.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第12期1473-1475,共3页
Chinese Journal of Pharmaceutical Analysis
