摘要
采用多重PCR对从流行过魏氏梭菌病的牧场粪便样品中分离魏氏梭菌的α、β、ε、ι 毒素基因进行了检测,确定了血清型。电泳成像显示,仅魏氏梭菌扩增出了特异性条带,其他对 照菌株无,说明该方法具有很高的特异性。通过对山东德州、枣庄、泰安、临沂、青岛等地养殖场 的418个样品分离的62个菌株的检测,检出率为14．8％,均为A型。研究确认,多重PCR是 魏氏梭菌血清型鉴别的一种快速、简便的研究方法,山东省流行型别与国外报道不完全一致。
For detection of C. Perfringens toxin types four pairs of primer for Alpha, Beta, Epsilon and Jota toxin gene were succeeded in designing and synthesizing according to the published sequence. The multiplex PCR was applied for the identification of toxin types of C. Perfringens from samples which were collected from earlier epidemic districts of Shandong province. The results showed that all isolated 62 samples （14.8%） were C. Perfringens toxin type A, that was Alpha toxin positive which is different from abroad reports.
出处
《家畜生态学报》
2005年第5期15-18,共4页
Journal of Domestic Animal Ecology
基金
山东省科委科技攻关项目
鲁科技[1999-43]Nr:993205905
2002
2
关键词
魏氏梭菌
血清型
多重PCR
流行病学
Clostridium Perfringens
toxin type
multiplex PCR
epidemiology
