摘要
采用紫外线诱变和紫外线复合氯化锂处理盐霉素生产菌株,利用含棕榈油的筛选平板,结合选育脂肪酶活力高的菌株,成功获得产量提高并适应棕榈油发酵的高产菌株Ae-4.棕榈油完全替代豆油摇瓶113 h发酵的产量是豆油发酵的88.7%(出发株为69.6%).进一步通过选育抗药性突变株获得了抗链霉素的高产突变株E4Lt-1(摇瓶发酵产量提高57.4%)和抗利福霉素的高产突变株Rift-1-2(摇瓶发酵产量提高44.9%).
A Streptomyces albus strain for industrial production of salinomycin is successfully mutated by UV radiation and screened on plates containing palm oil. The mutant Ae-4 which could produce high yield salinomycin using palm oil as carbon source is obtained according to its high lipase activity on the screening plate. By substituting palm oil for bean oil completely, the salinomycin yield of Ae-4 in shake flask is 88.7% of that fermented with bean oil, where as for the original strain, the value is only 69.6%. Further more E4Lt-1, a streptomycin -resistant mutant with enhanced （57.4% higher） salinomycin production and Rift-1-2, a rifampin-resistant mutant with enhanced （44.9% higher） salinomycin production are screened from Ae-4 by UV radiation.
出处
《郑州大学学报（理学版）》
CAS
2005年第4期92-95,共4页
Journal of Zhengzhou University:Natural Science Edition
关键词
白色链霉菌
盐霉素
紫外线诱变
抗药性
Streptomyces albus
salinomycin
UV radiation
drug resistance
