摘要
目的:建立全血中环孢素A(CsA)的RP-HPLC测定法,监测肾移植受者全血中CsA的C0、C2值.方法:RP-HPLC法色谱条件为:SB-C18柱,流动相为乙腈-甲醇-异丙醇-水(58:18:1:23),流速:1.4 mL·min-1,柱温70℃,检测波长208 nm.结果:CsA在51.25～1230μg·L-1范围内有良好的线性关系,r=0.999 3.绝对回收率为77.1%～90.2%,日内RSD≤10(n=6),日间RSD≤11.5(n=6).结论:此法简便、重现性好,可用于器官移植受者CsA的C0、C2的血药浓度监测.
OBJECTIVE To develop an RP-HPLC method for determining the C0 and C2 of cyclosporine A（CsA） in renal transplant patients whole blood. METHODS Detection was performed on SB-Cl8 column with acetonitrile-methanol-isopropanol-water （58：18： 1：23） as mobile phase. Detecting wavelength was 208 nm; The flow rate was 1.4 mL. min^-1 and column temperature was 70℃ RESULTS The calibration curve was linear in the concentrations ranged from 51.25 to 1 230 μg·L^-1（r= 0. 999 3）. Absolute recovery of CsA was 77. 1%～90. 2%, the inter-day RSD was less than 10% （n = 6） and the intra-day RSD was less than 11.5% （n = 6）. CONCLUSION The method is simple and reliable and can be applied to determine the C0 and C2 concentration of CsA in whole blood of patients receiving transplantation.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第11期1031-1033,共3页
Chinese Journal of Hospital Pharmacy
