摘要
用由247个株系组成的珍汕97B/密阳46重组自交系群体及其含207个分子标记的连锁图谱,在2002年和2003年分别测定亲本和重组自交系群体开花后10d和20d籽粒的淀粉分支酶的活性,检测到3个控制开花后10dQ酶活性的主效应QTL(quantitativetraitloci),联合贡献率为10%,其中qQ10-6与环境发生显著的互作;分别检测到5对和2对染色体区间对开花后10d、20dQ酶活性的影响具有加性×加性上位性作用,其中开花后10d的3对染色体区间具有显著的上位性×环境互作效应。由此可见,水稻籽粒Q酶活性相关基因的表达,受到环境因子的极大影响。
To make an genetic analysis of starch branching enzyme （Q enzyme） inheritance, which is important in catalyzing the formation of amylopectin and thereby raising the amylopectin/amylose ratio of starch in rice grains, a linkage map of 207 DNA markers were constructed by using 247 recombinant inbred lines derived from an indica-indica rice cross Zhenshan 97B/ Milyang46. In 2002 and 2003, the activities of starch branching enzyme of the parents and 247 RILs were measured 10 d and 20 d respectively after flowering （Table 1）. A total of 3 quantitative trait loci （QTLs） were detected to have significant additive effects on Q enzyme activities 10 d after flowering with 10% phenotypic variations explained for the three QTLs （Table 2, Fig. 1）. Meanwhile, qQlO-6 with significant QTL×environment interaction was detected. Epistasis analysis detected 5 and 2 significant additive-by-additive interactions for Q enzyme 10 d and 20 d respectively after flowering （Table 3）, and three pairs of QTLs 10 d after flowering with significant epistasis×environment interactions were detected, explaining 3% to 12% of the phenotypic variation. The results showed that the environmental factors had obvious effect on the gene expression of Q enzyme activity in rice grain. Meanwhile, the location of qQ10-6 in relation to that of QTLs for gelatinization temperature, gene loci involved in ADPG pyrophosphorylase and soluble starch synthase were discussed with the help of a genetic map.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2005年第6期631-636,共6页
Journal Of Plant Physiology and Molecular Biology
基金
国家"863"计划(No.2003AA207030)
浙江省重点项目(No.2003G10028)
中国博士后科学基金(No.2003034232)资助。~~
关键词
水稻
数量性状座位
淀粉分支酶(Q酶)
rice
quantitative trait loci
starch branching enzyme（Q enzyme）
