摘要
目的应用扫描电镜观察骨小梁微破裂形态及微骨痂的微观结构,探讨微骨痂的超微结构特征。方法取63周龄雌性SD大鼠第6腰椎椎体,沿冠状面切开,蒸馏水冲洗净骨髓腔后乙醇梯度脱水,表面喷金后应用扫描电镜观察,在600倍下观察骨小梁微破裂以及微骨痂的形态,在2000倍和10000倍下观察微骨痂的细微及超微结构。结果观察到微破裂长度约80μm,在骨微破裂一端靠近小梁边缘处,可见有微骨痂形成,微骨痂为一种疏松多孔的海绵状结构,其超微结构呈珊瑚礁形貌,由直径约0.2～0.5μm大小网状排列的骨小梁类似结构构成。结论在微骨痂修复微破裂过程中可能存在一种“超微小梁”结构,这种结构对于受损骨小梁承受应力以及修复均有利。
Objective To observe the microshape of microcracking and ultra-microstructure of microcallus on bone trabeculae with scanning electron microscopy. Methods The L6 vertebrae of 63-week-old female SD rat was coronarily sectioned. Subsequently, the bone marrow was carefully rinsed with distill water and dehydrated in graded ethanol. After coated with gold, observation of trabeculae was performed with scanning electron microscopy. Results The average length of microcracks was around 80 μm. The microcallus was detected near the edge of bone trabeculae on the extremity of microcrack. The microcallus was a kind of loose, spongy and coralloid structure and consisted of trabeculae-liked net in 0.2μm to 0.5 μm diameter. Conclusions A kind of structure named ultratrabeculae might appear during the healing process of microcracks which is beneficial to bear loading and repair the damaged trabeculae.
出处
《中国骨质疏松杂志》
CAS
CSCD
2005年第4期434-436,共3页
Chinese Journal of Osteoporosis
基金
国家自然科学基金项目(30400514)
卫生部临床学科重点建设项目(2004)
关键词
骨小梁微破裂
微骨痂
扫描电镜
超微小梁
超微结构
Bone trabeculae
Microcrack
Microcallus
Scanning electron microscopy
Ultra-trabeculae
