摘要
以肾细胞作材料,采用秋水仙素-低渗-空气干燥法、Howell的方法(银染)和Sumner的方法(C带),制作染色体标本。报道了星丽鱼(Astronotus ocellatus)、天使鱼(Pterophyllum scalare)的核型及银染和C带。结果显示,这两种热带淡水观赏鱼的2n均为48。星丽鱼的核型公式为2n=2m+26sm+16st+4t,NF=76,其染色体经快速银染后表明,在sm1的随体上出现银染位点;多数染色体的着丝点区均显示出一个深浅不同的C带,随体所在位置均深染。天使鱼具有异形性染色体,其核型公式为♀:2n=4m(X)+12sm+12st+20t,NF=64;♂:2n=3m(X)+12sm+12st+21t(Y),NF=63。银染点位于sm3的短臂末端和X染色体的短臂末端;多数染色体为着丝粒C带。在两种鱼中均未见到Ag-NORs联合现象。
Chromosome specimen of kidney cells was prepared by using colchicine-low concentration-air drying method, Howell＇s （1980） Ag-NORs staining method and Sumner＇s （1972） C-Banding staining method. The karyotype,Ag-NORs and C-Banding of two kinds of tropic freshwater fishes, Astronotus ocellatus and Pterophyllum scalare, were observed. The results showed that these two kinds of tropic freshwater fish have a chromosome number of 2n = 48. The karyotype of A. ocellatus was 2n = 2m ＋ 26 sm ＋ 16st ＋ 4t, NF = 76. The Ag-NOR staining pots could be observed on the satellites of sm1 ; lightly stained and darkly stained C-Banding were observed on centromeres of most chromosomes; and the satellites were darkly stained. P. scalare possessed sex chromosomes X and Y with a karyotype of ♀：2n =4m（X） ＋ 12 sm ＋ 12st ＋ 20t, NF = 64; and karyotype of ♂ ： 2n = 3m （X） ＋ 12 sm ＋ 12st ＋ 21t （Y）, NF = 63. Ag-NORs pots could be observed on the tips of short ann of sm3 and X chromosome. The type of C-Banding in most chromosomes was centrometric .There was no Ag-NORs combination in both fishes.
出处
《动物学杂志》
CAS
CSCD
北大核心
2005年第6期84-90,共7页
Chinese Journal of Zoology
基金
福建省自然科学基金(No.B0210012)
福建省教育厅科学基金(No.JA02183)
关键词
星丽鱼
天使鱼
染色体组型
银染
C带
Astronotus ocellatus
Pterophyllum scalare
Karyotype
Ag-NORs
C-Banding
