摘要
目的 研究丹参对缺氧缺血性新生大鼠脑皮质氧化还原因子-1 (redoxfactor-1,Ref-1)蛋白和凋亡细胞的影响。方法 将新生7日龄SD大鼠制成缺 氧缺血性脑损伤(HIBD)动物模型,采用免疫组织化学方法及原位缺口末端标记 (TUNEL)法分别观察假手术组、缺氧缺血组及丹参治疗组脑皮质Ref-1蛋白及神经 细胞凋亡变化。结果 与缺氧缺血组比较,丹参治疗组Ref-1蛋白表达Ref-1阳性细 胞数增加(由36．1±6．3上升至76．3±5．4,t=2．43,P<0．05);而凋亡细胞显著减 少(由68±2降至12±5,t=7．02,P<0．01)。结论 丹参作为自由基清除剂可能通 过促进缺氧缺血后脑神经细胞Ref-1蛋白的表达,从而抑制了细胞凋亡,为治疗新生 儿缺氧缺血性脑病提供新的理论依据。
[ Abstract ] Objective To study the effect of radix salivae miltiorrhizae （SM） on Redox factor-1 （Ref-1） protein and apoptosis of the cerebral cortex area in neonatal rats after hypoxic-ischemic brain damage （HIBD）. Methods The HIBD model was established with 7-day old SD neonatal rats. The expression of Ref-1 protein and apoptosis in the cerebral cortex area were determined by immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling（TUNEL） staining in sham operation group, HIBD group and SM group （treated by Compound Dan-shen） with intervals of 24h. Results Compared with the HIBD group, the expression of Ref-1 protein was increased （36. 1 ± 6. 3 vs. 76. 3 ± 5.4, t = 2.43 ,P 〈 0. 05 ） and apoptosis cells were decreased significantly （ 68±2 vs. 12±5, t =7.02 ,P 〈0.01 ） in SM group. Conclusions As free radical scavenge, SM can prevent neuron apoptosis after HIBD by enhancing expression of Ref-1 protein probably. It provides a new theoretic evidence for treating neonatal hypoxic-ischemic encephalopathy.
出处
《新生儿科杂志》
2005年第6期261-264,共4页
The Journal of Neonatology
关键词
丹参
缺氧缺血
脑皮质
氧化还原因子-1
凋亡
Radix salivae miltiorrhizae （SM）
Hypoxia-ischemia
Cerebral cortex
Redox factor-1
Apoptosis
