摘要
采用AtlantisTMC18分析柱(250mm×4.6mmID,5μm),以ResinHZ201强碱型阴离子交换树脂消除样品中普遍存在的NO3干扰,建立了一种反相高效液相色谱法测定蔬菜中草酸的分离与分析方法,在紫外220nm条件下检测,线性范围为0.5～300μg/ml,变异系数为1.4%(n=10),检测限(S/N=3)可达23ng/ml,用于蔬菜中草酸含量的检测实验结果表明,该方法具有灵敏、回收率高(达100.2%～101.9%)、干扰消除效果好,而且操作简便、成本低等优点。-
A method was established for the determination of oxalate in vegetables by Reversed Phase High Performance Liquid Chromatography with a reversed-phase AtlantisTM C 18 column（250mm ×4.6mm ID,5μm）as immobile phase. The general interfering factor NO3^- in samples was eliminated by using the powerful anion exchange resin - Resin HZ201. When detected with an UV detector at 220nm, the calibration curve showed a linear range of 0.5 -300μ g/ml with a coefficient of variation of 1.4%（n= 10）and a detection limit of 23 ng/ml（S/N=3）. It was found that the method was sensitive, with high percent recovery（from 100.2% to 101.9%）, little disturbance, convenient and low cost.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第12期172-175,共4页
Food Science
基金
国家自然科学基金资助项目(40371067)
关键词
反相高效液相色谱
强碱型阴离了交换树脂
干扰
蔬菜
草酸
reversed phase high performance liquid chromatography
strong base type anion- exchange resin
disturbance
vegetables： oxalate
