摘要
目的:观察活血化瘀中药长骨液对骨延长动物模型骨延长区的组织学变化,以及对成骨作用的影响。方法:实验于2001-02-05在成都军区昆明总医院实验动物中心实验实完成。54只新西兰大白兔造成左胫骨中段骨延长模型,并随机分为对照组和实验组,每组各27只。实验组于术后第2天开始喂含中药长骨液(由丹参、土鳖虫、骨碎补等组成)的颗粒饲料(每只按7.5mL/kg·d标准加入),对照组喂普通饲料。于延长7d、延长结束后0,7,14,21,30,50,60和70d,将两组各处死动物3只,切取延长区新骨组织,按常规做成光镜切片,在光镜下观察延长区新骨再生与成熟情况。结果:两组各有27只动物纳入结果分析。①两组胫骨中段骨延长情况:两组延长长度差异无显著性意义(P>0.05)。②各组胫骨中段骨延长的组织学变化:实验组在延长结束时,增厚的骨膜上有大量的毛细血管增生,可见大量的间充质细胞,成纤维细胞增生、活跃;延长结束后1～3周,骨膜下、骨小梁周围成骨细胞较多,且成骨活动较对照组更为活跃;延长结束后30d,延长区假性生长板比对照组提前消失。结论:长骨液通过促进骨延长区血管增生、改善局部的血液循环,促进骨生成细胞和破骨细胞数量的增加和功能的活跃,加速假性生长板成骨,来明显促进骨延长区新骨的形成与成熟。
AIM： To observe the effects of bone growth fluid on histological changes in bone elongation region of animal models and osteogenesis.
METHODS： The experiment was conducted at the laboratory of experimental animal center of Kunming General Hospital of Chengdu Military Area Command during February 2001. Bone lengthening model was made in the middle segment of fifty-four New Zealand rabbit tibia. The rabbits were assigned randomly into control group and experimental group with 27 in each group. The granules feed （7.5 mL/kg once a day） of bone growth fluid （composed of dan-shen root, ground beetle, fortune＇s drynaria rhizome, etc.） was feed into the rabbits in the experimental group from the 2^rd day after operation. Those in the control group were feed with common feed. Three animals were killed in every group, resnectively at elongation for 7 days, at the time of elongation, at 7 days, 14 oays, 21 days, 30 days, 50 days, 60 days and 70 days after elongation. New bone tissue at elongation region was gained, and slices of light microscope were made according to routine. Regeneration and maturity of new bone at elongation were observed under light microscope.
RESULTS： Twenty-seven animals in each group were involved in the result analysis. ① Bone elongation at middle segment of tibia in the two groups： The length of elongation in the two groups had insignificant difference （P 〉 0.05）. ② Histological changes of bone elongation in middle segment of tibia in every group： At the end of elongation there were lots of proliferation of blood capillary in thickening periosteum, showing many mesenchymal cells with proliferative and active fibroblasts in the experimental group. From 1 to 3 weeks after the end of elongation, many osteoblasts appeared around subperiosteum and bone trabecula. Osteogenesis activity was more active than that in the control group. At 30 days after the end of elongation, sham growth plate in elongation region disappeared earlier than that in the control group.
CONCLU
出处
《中国临床康复》
CSCD
北大核心
2005年第46期60-61,T0004,共3页
Chinese Journal of Clinical Rehabilitation
基金
云南省应用基础研究基金项目(批准号98C109M)~~
