摘要
目的:评价聚乳酸、聚乙醇酸及其共聚物的细胞毒性,为临床应用提供依据。方法:实验于2004-01/06在南方医科大学中心实验室完成。实验材料由暨南大学化学系提供。聚乳酸类材料,聚乙醇酸类材料,聚乳酸、聚乙醇酸的共聚物实验用细胞系:小鼠胚胎成纤维细胞购自中国科学院细胞库。采用能快速评定细胞增殖率和细胞毒性的比色分析方法--噻唑蓝比色法,分别检测对聚羟基乙酸和聚乳酸的共聚物细颗粒对小鼠胚胎成纤维细胞相对增殖率的影响,评价其细胞毒性。细胞毒性分级评估标准:0级,细胞相对增殖率≥100%;1级,细胞相对增殖率≥80%;2级,细胞相对增殖率≥50%;3级,细胞相对增殖率≥30%;4级,细胞相对增殖率≥0。结果:聚乳酸类增殖率94.1%,细胞毒性分级为1级;聚乙醇酸类增殖率94.4%,细胞毒性分级为1级;聚乳酸、聚乙醇酸的共聚物增殖率92.4%,细胞毒性分级为1级。结论:聚乳酸、聚乙醇酸及其共聚物无细胞毒性,可临床应用。
AIM： To assess the cytotoxicity of polyactic acid （PLA）, polyglycolic acid （PGA） and its copolymers and provide evidence for clinical application.
METHODS： The experiment was preformed at central lab of Southern Medical University from January to June 2004. The experimental materials were provided by department of chemistry of Jinan University： polyactic acid （PLA）, polyglycolic acid （PGA） and their copolymers. The cell series： embryo fibroblasts of mouse were come from cell storeroom of Chinese Academy of Sciences. The effects of homoconjugate of polyglycolic acid and PLA on the relative proliferation rate of embryo fibroblasts were detected by MTT-assay that could assess the proliferation rate and cytotoxicity rapidly, and to evaluate their cytotoxicity. Evaluating criteria on cytotoxicity： zero grade as relative proliferation rate of cells was at least 100%; first grade as relative proliferation rate of cells was at least 80%; second grade as relative proliferation rate of cells was at least 50%; third grade as relative proliferation rate of cells was at least 30%; fourth grade as relative proliferation rate of cells was at least 0.
RESULTS： Cell relative proliferation rate of PLA was 94.1%, cytotoxicity was first grade; cell relative proliferation rate of PGA was 94.4%, first grade; cell relative proliferation rate of their homoconjugate was 92.4%, first grade.
CONCLUSION： PLA, PGA and its copolymers have no cytotoxicity and can be used in clinic.
出处
《中国临床康复》
CSCD
北大核心
2005年第46期46-47,共2页
Chinese Journal of Clinical Rehabilitation
