摘要
目的、方法：用高效液相色谱分析、比较豚鼠咽鼓管和肺表面活性物质中磷脂的组成成分和含量，为人工合成咽鼓管表面活性物质，预防和治疗中耳炎提供理论依据。结果：咽鼓管和肺表面活性物质中均检测出了磷脂酰胆碱（ＰＣ）、磷脂酰肌醇（ＰＩ）、磷脂酰丝氨酸（ＰＳ）、磷脂酸（ＰＡ）、磷脂酰乙醇胺（ＰＥ）、神经鞘磷脂（ＳＰ），每４只咽鼓管中各磷脂含量（ｇ）及所占百分比：ＰＥ：１２３．４６４０．６６，（４２８）％；ＳＰ：６７．９５１７．９４，（２１４）％；ＰＳ：４８．５７土１８．８４，（１６４％）；ＰＣ：３２．６０１３．１７，（１１４）％；ＰＩ：２２．９４５．７３，（８士２）％；ＰＡ：２．９０士０．００，（１０．３）％．而１个肺中：ＰＣ：５６３．８２士７４．５，（６８土４）％；ＰＥ：９５．９２士２０．３０，（１１士２）％；Ｐｌ：６８．３３士１４．６３，（８士１）％；ＰＳ：５１．４３士１４．２６，（６２）％；ＳＰ：３４．０４土６．１３，（４士１）％：ＰＡ：１６．８３ｌ．６１，（２土０）％。结论：咽鼓管表面活性物质中磷腊成分含量不同于肺，提示体内不同部位的表面活性物质可能各有特殊的功能作用。
Objective and Methods: To analyse and compare the biochemical component and content of phospholipids of surface active substance between eustachian tube and lung in guinea pig by the high performance liquid chromatographty (HPLC). The present study was undertaken to produce rationale for artificial synthesizing effective eustachian tube surfactant to prevent and treat aural barotrauma. Results: Phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidic acid (PA), sphingophospholipid (SP) were detected. The content (μg/four ear) and percentage of phospholipid in eustachian tube surfactance: PE: 123.46±40.66, (42±8)%; SP: 67.95±17.94, (21±4)%; PS: 48.57±18.84, (16±4%); PC: 32.60±13.17, (11±4)%; PI: 22.94±5.73, (8±2)%; PA: 2.90±0.00, (1±0.3)%. In comparison, in pulmonary lavage (μg/one lung): PC: 563.82±74.5, (68±4)%; PE: 95.92±20.30, (11±2)%; PI: 68.33±14.63, (8±1)%; PS: 51.43±14.26, (6±2)%; SP: 34.04±6.13, (4±1)%; PA: 16.83±1.61, (2±0)%. Conclusion: The phospholipid composition of eustachian tube surfactants are different from that of pulmonary surfactants, suggesting that the surfactants located in the different organs have their specific function.
出处
《第四军医大学学报》
1996年第4期251-253,共3页
Journal of the Fourth Military Medical University
关键词
咽鼓管
表面活性物质
磷脂
eustachian tube surfactance phospholipid
