摘要
天然香兰素一直是香精香料行业关注的重点。本文介绍一株链霉菌L1936,它能以阿魏酸和香兰素为唯一碳源生长,且能将阿魏酸转化为香兰素。并发现阿魏酸是生产香兰素的一种非常好的底物,添加量可达12g/L。测试了链霉菌L1936对阿魏酸的转化能力,能够将6g/L阿魏酸转化为2.02g/L香兰素,相应的摩尔转化率为42.97%。而且此株链霉菌具有一种与其它菌株完全不同的代谢流。在转化阿魏酸时,当香草酸的积累量达到200mg/L时,就开始积累香兰素作为代谢的主要过量合成产物。流加两次底物阿魏酸后,产物浓度达到4.38g/L,相应的摩尔转化率为44.58%。
Natural vanillin is of high interest to the flavor and fragrance industry. The present work describes that microbial strain Streptomyces L1936 isolated from microorganisms, capable of growing on femlic acid or vanillin as the sole carbon source. Strain L1936 can produce high amounts of vanillin when grown in the presence of ferulic acid. Femlic acid was found to be an excellent precursor for the conversion to vanillin, as doses of several 4.38g/L could be fed . This strain could transform 6g/L ferulic acid to produce 2.02g/L vanillin with a molar yield of 42.97 %. A completely different metabolic flux was observed with Streptomyces L1936. During the metabolism of femlic acid , this strain accumulated vanillin acid only to a level of around 200mg/L and then started to accumulated vanillin as the principal metabolic product. After femlic acid was sequentially added into the medium for two times, the concentration of vanillin reached 4.38g/L with a molar yield of 44.58%.
出处
《吉林农业科技学院学报》
2005年第1期1-4,共4页
Journal of Jilin Agricultural Science and Technology University
关键词
链霉菌
生物转化
阿魏酸
香兰素
Streptomyces
bioconversion
ferulic acid
vanillin
