摘要
以低能氮离子束为诱变源,对甘草种进行注入处理后,以萌发的幼苗为材料对其在干旱胁迫条件下保护酶活性及MDA含量变化进行研究。结果表明:注入后甘草种的存活率呈现马鞍形曲线变化,其中在4.1×1016N+/cm2剂量处达到鞍点,在剂量4.6×1016N+/cm2处存活率高于对照。在干旱胁迫下,随着胁迫时间的延长,离子束注入后萌发的幼苗MDA含量明显低于对照。酶活结果显示,在干旱胁迫后期,剂量4.6×1016N+/cm2处理后的甘草幼苗SOD和CAT酶活性都高于对照,说明该注入剂量处理甘草种有助于提高甘草幼苗的抗旱性能,SOD同工酶结果也证明该剂量的离子注入对干旱胁迫下同工酶谱带有所影响。
The effect of N^＋ ion implantation on the antioxidative system （SOD,CAT）and MDA content of liquorice seedlings（Glycyrrhiza uralensis Fisch ）under PEG-induced drought stress was investigated in this study. The results showed survival ratio of liquorice seeds exhibited saddle-shaped curve. When the dose was 4.6×10^16N^＋/cm^2 , the survival ratio was the lowest, while the survival ratio was higher than that of control when the dose increased to 4.6×10^16N^＋/cm^2. MDA measurement pointed out that the contents were significantly lower in seedlings irradiated by N^＋ ion beams of 4.6×10^16N^＋/cm^2 dose when compared to control. The data also showed that SOD and CAT activity of irradia- ted seedlings by dose of 4.6×10^16N^＋/cm^2 ion beam were higher than control, indicating that this implantation was useful in enhancement of drought resistance of liquorice seedlings. Result of SOD isoform pattern also demonstrates the biological effects of N d^＋ ion implantation.
出处
《激光生物学报》
CAS
CSCD
2005年第6期442-446,共5页
Acta Laser Biology Sinica
基金
国家"863"计划(2002AA327070)
(2004AA326060)资助项目
关键词
离子注入
甘草幼苗
干旱胁迫
保护酶
MDA
ion beam implantation
liquorice seedlings
drought stress
protective enzymes
MDA
