摘要
目的研究血管紧张素转换酶(ACE)和血管紧张素原(AGT)基因多态性与冠心病的关系。方法对72例冠心病(CAD)患者用PCR法进行ACE基因插入/缺失多态和用PCR-RFLP法检测AGT基因多态性,并与50例健康对照组比较。结果CAD组ACE的DD基因频率与对照组相比无显著差异。CAD组ACG中T235M频率与对照组相比有差异(P=0.048),联合基因多态分析,CAD组合并ACE-DD+AGT-TT基因型频率显著高于对照组(P=0.029)。结论ACE-DD基因型频率与AGT-TT基因型频率在CAD发病中具有协同作用。
Objective To investigate the relationship between the genetic polymorphisms of angiotensin system and the risk of coronary artery disease （CAD）. Methods The insertion/deletion of angiotensin converting enzyme （ACE） and polymorphisms of angiotensinogen （AGT） gene were analyzed by using polymerase chain reaction （PCR） and PCR- restriction fragment length polymorphlsm（ PCR - RFLP） in 72 patients with CAD and 50 healthy controls. Results There was no significant difference in the gene frequency of ACE - DD between the patients with CAD and the controls（ P 〉 0. 05）. The gene frequencies of ACG - T235M and ACE - DD plus AGT - TIP were s!gnificantly higher in the patients with CAD than in the controls respectively （ all P 〈 0. 05 ）. Conclusion The gene frequency of ACE - DD and AGT - TF may have a synergistic effect on the risk of CAD.
出处
《中国医师杂志》
CAS
2005年第12期1593-1595,共3页
Journal of Chinese Physician
基金
广东省珠海市科技局资助项目(200301031)
关键词
冠心病
血管紧张素原
血管紧张素转换酶
基因多态性
Coronary artery disease
Angiotensinogen
Angiotensin converting enzyme
Gene polymorphism
