摘要
目的探讨人早孕期外周血及蜕膜淋巴细胞协同刺激分子表达水平在母-胎免疫调节中的作用。方法以15例正常育龄妇女黄体期外周血为对照,应用流式细胞仪分析15例正常人早孕期外周血及蜕膜淋巴细胞CD28和CTLA-4的表达水平。结果各组淋巴细胞几乎不表达表面CTLA-4分子;而表达细胞内CTLA-4分子(CTLA-4i)。蜕膜组织淋巴细胞CTLA-4i+/CD28+的比例显著高于早孕期及黄体期外周血。早孕期与黄体期外周CTLA-4i+/CD28+的百分率比较无显著性差异。结论母胎界面局部高表达细胞内CTLA-4在母-胎免疫耐受中起重要作用。
Objective：To probe into the role of expressions of the costimulatory molecules on peripheral blood and deeidual lympho- eytes of early pregnancy in matemo-fetal immune regulation.Methods：Fifteen women of the early pregnancy were analyzed by direct immunofluorescence flow cytometry for expression of CD28 and CILA-4 on lymphocytes of peripheral blood and decidua, and 15 healthy fertile women of luteal phase were recruited as control. Results： All the peripheral or decidual lymphocytes hardly expressed surface CTLA-4, but expressed intracellular CTLA-4（CTLA-4i）.The expression ratios of CTLA-4i^＋/CD28^＋ on lymphocyte increased significantly in decidun compared to peripheral blood of the early pregnancy and luteal phase. However there were no significant difference in the ratio of CTLA-4i^＋/CD28^＋ on peripheral lymphocyte between the early pregnancy and luteal phase. Conclusion：The hyper-expression of intracellular CTLA-4 in the decidual lymphocyte could play an important role in matemo-fetal immune regulation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2005年第12期944-946,共3页
Chinese Journal of Immunology
基金
国家自然科学基金(39770773
30200299
30470264)
复旦大学"985工程"项目(985863)资助
关键词
早期妊娠
蜕膜淋巴细胞
协同刺激分子
Early pregnancy
Decidual lymphocyte
Costimulatory molecules
