摘要
目的应用酶联免疫吸附试验(ELISA)检测唾液中抗戊型肝炎病毒(HEV)-IgM,评价其用于戊型肝炎早期诊断的可行性。方法应用东南大学基因工程疫苗研究所建立的HEV-IgM抗体ELISA检测方法,检测77例急性戊型肝炎患者、63例非戊型肝炎患者及64例正常人的唾液和血清样本中抗HEV-IgM,将唾液和血清两者的检测结果加以比较,并观察标本采集时间对检测结果的影响。结果77份急性戊型肝炎患者血清与唾液抗HEV-IgM阳性符合率为87%;在64份正常对照组及63份其他患者对照组中,血清与唾液抗HEV-IgM均阴性,阴性符合率为100%。标本采集时间对唾液和血清检测结果的相关性无显著影响。结论唾液标本用于抗HEV-IgM检测,特异性好,敏感性较高,具有无创伤、容易采集、操作简便等优点,在特殊情况下可代替血清用于戊型肝炎的诊断,尤其适用于戊型肝炎流行时HEV近期感染的血清流行病学调查。
Objective To explore the feasibility of early diagnosis for hepatitis E by detection of anti-hepatitis E virus （HEV） IgM in saliva samples. Methods Comparable samples of serum and saliva collected from 77 patients suffered from hepatitis E, 63 patients with non-HEV infection and 64 health people were examined for anti-HEV IgM by an ELISA which was established on the basis of HEV multiple-genotype recombinant antigens. The positive and negative rates were compared between the serum and saliva samples with statistical analysis. Influence of sampling time in the duration of the disease on the results of anti-HEV IgM detection was investigated. Results Consistent positive rate of anti-HEV IgM was 87% between the serum samples and saliva samples collected from 77 patients with hepatitis E. In the patients with non-HEV infection and the health people, no one was positive for anti-HEV IgM both in serum and in saliva. Therefore, the total consistency was 95% between the two samples. In addition, the sampling time did not affect the relativity of anti-HEV positive rates between serum and saliva specimens. Conclusions Comparing with serum specimen, sampling saliva has many advantages, such as harmlessness and easy collection. Detection of anti-HEV IgM by using saliva sample is feasible for early diagnosis of hepatitis F2, especially, for seroepidemiological investigation of recent HEV infection during HEV epidemic.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2005年第5期342-344,共3页
Chinese Journal of Infectious Diseases
基金
江苏省卫生厅医学科技发展基金资助项目(H200115)
